Chromatography Paper Strip Sampling of Enteric Adenoviruses Type 40 and 41 Positive Stool Specimens

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2005 Feb 12

PMID 15705203

Citations 15

Authors

Kalina T Zlateva

Piet Maes

Mustafizur Rahman

Marc Van Ranst

Affiliations

Soon will be listed here.

Abstract

Background: The enteric subgroup F adenoviruses type 40 (Ad40) and 41 (Ad41) are the second most important cause of acute infantile gastroenteritis after rotaviruses. Repeated community outbreaks have been associated with antigenic changes among the Ad40 and Ad41 strains due to host immune pressure. Therefore large field epidemiological surveys and studies on the genetic variations in different isolates of Ad40 and Ad41 are important for disease control programs, the design of efficient diagnostic kits and vaccines against subgroup F adenoviruses. A novel method using sodium dodecyl sulphate SDS/EDTA-pretreated chromatography paper strips was evaluated for the collection, storage and shipping of Ad40/41 contaminated stool samples.

Results: This study shows that adenoviral DNA can be successfully detected in the filter strips by PCR after four months storage at -20 degrees C, 4 degrees C, room temperature (20-25 degrees C) and 37 degrees C. Furthermore no adenoviral infectivity was observed upon contact with the SDS/EDTA-pretreated strips.

Conclusions: Collecting, storing and transporting adenovirus type 40 and 41 positive stool samples on SDS/EDTA-pretreated chromatography filter strips is a convenient, biosafe and cost effective method for studying new genome variants and monitoring spread of enteric adenovirus strains during outbreaks.

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References

Carnevale S, Velasquez J, Labbe J, Chertcoff A, Cabrera M, Rodriguez M . Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks. Clin Diagn Lab Immunol. 2000; 7(3):504-6. PMC: 95902. DOI: 10.1128/CDLI.7.3.504-506.2000. View

Beck I, Drennan K, Melvin A, Mohan K, Herz A, Alarcon J . Simple, sensitive, and specific detection of human immunodeficiency virus type 1 subtype B DNA in dried blood samples for diagnosis in infants in the field. J Clin Microbiol. 2001; 39(1):29-33. PMC: 87674. DOI: 10.1128/JCM.39.1.29-33.2001. View

de Swart R, Nur Y, Abdallah A, Kruining H, El Mubarak H, Ibrahim S . Combination of reverse transcriptase PCR analysis and immunoglobulin M detection on filter paper blood samples allows diagnostic and epidemiological studies of measles. J Clin Microbiol. 2001; 39(1):270-3. PMC: 87713. DOI: 10.1128/JCM.39.1.270-273.2001. View

Allard A, Albinsson B, Wadell G . Rapid typing of human adenoviruses by a general PCR combined with restriction endonuclease analysis. J Clin Microbiol. 2001; 39(2):498-505. PMC: 87765. DOI: 10.1128/JCM.39.2.498-505.2001. View

Rahman M, Goegebuer T, De Leener K, Maes P, Matthijnssens J, Podder G . Chromatography paper strip method for collection, transportation, and storage of rotavirus RNA in stool samples. J Clin Microbiol. 2004; 42(4):1605-8. PMC: 387597. DOI: 10.1128/JCM.42.4.1605-1608.2004. View

Wollants E, Maes P, Thoelen I, Vanneste F, Rahman M, Van Ranst M . Evaluation of a norovirus sampling method using sodium dodecyl sulfate/EDTA-pretreated chromatography paper strips. J Virol Methods. 2004; 122(1):45-8. DOI: 10.1016/j.jviromet.2004.08.001. View

Yolken R, Lawrence F, Leister F, Takiff H, Strauss S . Gastroenteritis associated with enteric type adenovirus in hospitalized infants. J Pediatr. 1982; 101(1):21-6. DOI: 10.1016/s0022-3476(82)80173-x. View

Chiba S, Nakata S, Nakamura I, Taniguchi K, URASAWA S, Fujinaga K . Outbreak of infantile gastroenteritis due to type 40 adenovirus. Lancet. 1983; 2(8356):954-7. DOI: 10.1016/s0140-6736(83)90463-4. View

KIDD A . Genome variants of adenovirus 41 (subgroup G) from children with diarrhoea in South Africa. J Med Virol. 1984; 14(1):49-59. DOI: 10.1002/jmv.1890140108. View

10.

Uhnoo I, Wadell G, Svensson L, Johansson M . Importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. J Clin Microbiol. 1984; 20(3):365-72. PMC: 271331. DOI: 10.1128/jcm.20.3.365-372.1984. View

11.

KIDD A, Berkowitz F, Blaskovic P, Schoub B . Genome variants of human adenovirus 40 (subgroup F). J Med Virol. 1984; 14(3):235-46. DOI: 10.1002/jmv.1890140307. View

12.

Yolken R, Franklin C . Gastrointestinal adenovirus: an important cause of morbidity in patients with necrotizing enterocolitis and gastrointestinal surgery. Pediatr Infect Dis. 1985; 4(1):42-7. View

13.

Madeley C . The emerging role of adenoviruses as inducers of gastroenteritis. Pediatr Infect Dis. 1986; 5(1 Suppl):S63-74. DOI: 10.1097/00006454-198601001-00012. View

14.

Ahluwalia G, Klisko B, Hammond G . Prevalent enteric adenovirus variant not detected by commercial monoclonal antibody enzyme immunoassay. J Clin Microbiol. 1990; 28(12):2797-801. PMC: 268276. DOI: 10.1128/jcm.28.12.2797-2801.1990. View

15.

Shinozaki T, Araki K, Fujita Y, Kobayashi M, Tajima T, Abe T . Epidemiology of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children in the Tokyo area. Scand J Infect Dis. 1991; 23(5):543-7. DOI: 10.3109/00365549109105175. View

16.

Van R, Wun C, ORyan M, Matson D, Jackson L, Pickering L . Outbreaks of human enteric adenovirus types 40 and 41 in Houston day care centers. J Pediatr. 1992; 120(4 Pt 1):516-21. DOI: 10.1016/s0022-3476(05)82477-1. View

17.

Unicomb L . Seroprevalence of enteric and nonenteric adenoviruses in Bangladesh. J Clin Microbiol. 1992; 30(10):2733-4. PMC: 270510. DOI: 10.1128/jcm.30.10.2733-2734.1992. View

18.

Fiscus S, Brambilla D, Grosso L, Schock J, Cronin M . Quantitation of human immunodeficiency virus type 1 RNA in plasma by using blood dried on filter paper. J Clin Microbiol. 1998; 36(1):258-60. PMC: 124847. DOI: 10.1128/JCM.36.1.258-260.1998. View

19.

Abe K, Konomi N . Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature. J Clin Microbiol. 1998; 36(10):3070-2. PMC: 105116. DOI: 10.1128/JCM.36.10.3070-3072.1998. View

20.

Moore P, Steele A, Lecatsas G, Alexander J . Characterisation of gastro-enteritis-associated adenoviruses in South Africa. S Afr Med J. 1999; 88(12):1587-92. View