Reliability of the E-test Method for Detection of Colistin Resistance in Clinical Isolates of Acinetobacter Baumannii

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2005 Feb 8

PMID 15695701

Citations 39

Authors

L A Arroyo

A Garcia-Curiel

M E Pachon-Ibanez

A C Llanos

M Ruiz

J Pachon

J Aznar

Affiliations

Soon will be listed here.

Abstract

We compared the E-test to the broth microdilution method for testing the susceptibility of 115 clinical isolates of Acinetobacter baumannii to colistin. Twenty-two (19.1%) strains were resistant to colistin and 93 (80.8%) strains were susceptible according to the reference broth microdilution method. A categorical agreement of 98.2% was found, with only two (1.7%) very major errors. Agreement within 1 twofold dilution between the E-test and the broth microdilution was 16.5%. Complete agreement was found for the strains for which MICs fell within the range of 0.25 to 1 microg of colistin/ml. However, there was poor concordance, particularly in extreme dilutions with higher MICs by the E-test method.

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