Binding of DNA to Alpha/beta-type Small, Acid-soluble Proteins from Spores of Bacillus or Clostridium Species Prevents Formation of Cytosine Dimers, Cytosine-thymine Dimers, and Bipyrimidine Photoadducts After UV Irradiation

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1992 May 1

PMID 1569018

Citations 13

Authors

H Fairhead

P Setlow

Affiliations

Soon will be listed here.

Abstract

Small, acid-soluble proteins (SASP) of the alpha/beta-type from spores of Bacillus and Clostridium species bind to DNA; this binding prevents formation of cyclobutane-type thymine dimers upon UV irradiation, but promotes formation of the spore photoproduct, an adduct between adjacent thymine residues. alpha/beta-Type SASP also bound to poly(dG).poly(dC) and poly(dA-dG).poly(dC-dT). While UV irradiation of poly(dG).poly(dC) produced cyclobutane-type cytosine dimers as well as fluorescent bipyrimidine adducts, the yields of both types of photoproduct were greatly reduced upon irradiation of alpha/beta-type SASP-poly(dG).poly(dC) complexes. UV irradiation of poly(dA-dG).poly(dC-dT) produced a significant amount of a cyclobutane dimer between cytosine and thymine, as well as a 6-4 bipyrimidine adduct. Again, binding of alpha/beta-type SASP to poly(dA-dG).poly(dC-dT) greatly reduced formation of these two photoproducts, although formation of the cytosine-thymine analog of the spore photoproduct was not observed. These data provide further evidence for the dramatic change in DNA structure and photoreactivity which takes place on binding of alpha/beta-type SASP and suggest that binding of these proteins to DNA in vivo prevents formation of most deleterious photoproducts upon UV irradiation.

Citing Articles

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References

Setlow P . Purification and properties of some unique low molecular weight basic proteins degraded during germination of Bacillus megaterium spores. J Biol Chem. 1975; 250(20):8168-73. View

Franklin W, Lo K, Haseltine W . Alkaline lability of fluorescent photoproducts produced in ultraviolet light-irradiated DNA. J Biol Chem. 1982; 257(22):13535-43. View

Tang M, Htun H, Cheng Y, Dahlberg J . Suppression of cyclobutane and mean value of 6-4 dipyrimidines formation in triple-stranded H-DNA. Biochemistry. 1991; 30(28):7021-6. DOI: 10.1021/bi00242a030. View

Patrick M . Studies on thymine-derived UV photoproducts in DNA--I. Formation and biological role of pyrimidine adducts in DNA. Photochem Photobiol. 1977; 25(4):357-72. DOI: 10.1111/j.1751-1097.1977.tb07355.x. View

Setlow B, Sun D, Setlow P . Interaction between DNA and alpha/beta-type small, acid-soluble spore proteins: a new class of DNA-binding protein. J Bacteriol. 1992; 174(7):2312-22. PMC: 205853. DOI: 10.1128/jb.174.7.2312-2322.1992. View

Tovar-Rojo F, Setlow P . Effects of mutant small, acid-soluble spore proteins from Bacillus subtilis on DNA in vivo and in vitro. J Bacteriol. 1991; 173(15):4827-35. PMC: 208162. DOI: 10.1128/jb.173.15.4827-4835.1991. View

Nicholson W, Setlow B, Setlow P . Ultraviolet irradiation of DNA complexed with alpha/beta-type small, acid-soluble proteins from spores of Bacillus or Clostridium species makes spore photoproduct but not thymine dimers. Proc Natl Acad Sci U S A. 1991; 88(19):8288-92. PMC: 52493. DOI: 10.1073/pnas.88.19.8288. View

Nicholson W, Setlow B, Setlow P . Binding of DNA in vitro by a small, acid-soluble spore protein from Bacillus subtilis and the effect of this binding on DNA topology. J Bacteriol. 1990; 172(12):6900-6. PMC: 210809. DOI: 10.1128/jb.172.12.6900-6906.1990. View

Setlow P . Small, acid-soluble spore proteins of Bacillus species: structure, synthesis, genetics, function, and degradation. Annu Rev Microbiol. 1988; 42:319-38. DOI: 10.1146/annurev.mi.42.100188.001535. View

10.

Setlow B, Setlow P . Thymine-containing dimers as well as spore photoproducts are found in ultraviolet-irradiated Bacillus subtilis spores that lack small acid-soluble proteins. Proc Natl Acad Sci U S A. 1987; 84(2):421-3. PMC: 304219. DOI: 10.1073/pnas.84.2.421. View

11.

Francesconi S, Macalister T, Setlow B, Setlow P . Immunoelectron microscopic localization of small, acid-soluble spore proteins in sporulating cells of Bacillus subtilis. J Bacteriol. 1988; 170(12):5963-7. PMC: 211716. DOI: 10.1128/jb.170.12.5963-5967.1988. View

12.

Sarma M, Gupta G, Sarma R . 500-MHz 1H NMR study of poly(dG).poly(dC) in solution using one-dimensional nuclear Overhauser effect. Biochemistry. 1986; 25(12):3659-65. DOI: 10.1021/bi00360a028. View

13.

Setlow R, Carrier W, Bollum F . Pyrimidine dimers in UV-irradiated poly dI:dC. Proc Natl Acad Sci U S A. 1965; 53(5):1111-8. PMC: 301381. DOI: 10.1073/pnas.53.5.1111. View

14.

Lindsay J, Murrell W . A comparison of UV induced DNA photoproducts from isolated and non-isolated developing bacterial forespores. Biochem Biophys Res Commun. 1983; 113(2):618-25. DOI: 10.1016/0006-291x(83)91771-0. View

15.

Lippke J, Gordon L, Brash D, Haseltine W . Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences. Proc Natl Acad Sci U S A. 1981; 78(6):3388-92. PMC: 319573. DOI: 10.1073/pnas.78.6.3388. View

16.

Donnellan Jr J, Setlow R . Thymine Photoproducts but not Thymine Dimers Found in Ultraviolet-Irradiated Bacterial Spores. Science. 1965; 149(3681):308-10. DOI: 10.1126/science.149.3681.308. View

17.

Mason J, Setlow B, Waites W, Setlow P . Purification and amino acid sequence of two small, acid-soluble proteins from Clostridium bifermentans spores. FEMS Microbiol Lett. 1989; 52(1-2):139-43. DOI: 10.1016/0378-1097(89)90185-7. View

18.

Mason J, Setlow P . Essential role of small, acid-soluble spore proteins in resistance of Bacillus subtilis spores to UV light. J Bacteriol. 1986; 167(1):174-8. PMC: 212857. DOI: 10.1128/jb.167.1.174-178.1986. View

19.

Glickman B, Schaaper R, Haseltine W, Dunn R, Brash D . The C-C (6-4) UV photoproduct is mutagenic in Escherichia coli. Proc Natl Acad Sci U S A. 1986; 83(18):6945-9. PMC: 386627. DOI: 10.1073/pnas.83.18.6945. View

20.

Setlow R, Carrier W . Pyrimidine dimers in ultraviolet-irradiated DNA's. J Mol Biol. 1966; 17(1):237-54. DOI: 10.1016/s0022-2836(66)80105-5. View