Repair of Ultraviolet Light Damage in Saccharomyces Cerevisiae As Studied with Double- and Single-stranded Incoming DNAs

Overview

Journal Curr Genet

Specialty Genetics

Date 1992 Feb 1

PMID 1568259

Authors

D Keszenman-Pereyra

K Hieda

Affiliations

Soon will be listed here.

Abstract

Purified double- and single-stranded DNAs of the autonomously replicating vector M13RK9-T were irradiated with ultraviolet light (UV) in vitro and introduced into competent whole cells of Saccharomyces cerevisiae. Incoming double-stranded DNA was more sensitive to UV in excision repair-deficient rad2-1 cells than in proficient repair RAD+ cells, while single-stranded DNA exhibited high sensitivity in both host cells. The results indicate that in yeast there is no effective rescue of UV-incoming single-stranded DNA by excision repair or other constitutive dark repair processes.

References

Mennigmann H . Photoreactivation of bacteriophage phi chi 174. Int J Radiat Biol Relat Stud Phys Chem Med. 1975; 27(4):313-23. DOI: 10.1080/09553007514550281. View

Ono J, Shimazu Y . Ultraviolet reactivation of a bacteriophage containing a single-stranded deoxyribonucleic acid as a genetic element. Virology. 1966; 29(2):295-302. DOI: 10.1016/0042-6822(66)90036-5. View

Keszenman-Pereyra D, Hieda K . A colony procedure for transformation of Saccharomyces cerevisiae. Curr Genet. 1988; 13(1):21-3. DOI: 10.1007/BF00365751. View

Jansz H, Pouwels P, VAN ROTTERDAM . SENSITIVITY TO ULTRAVIOLET LIGHT OF SINGLE- AND DOUBLE-STRANDED DNA. Biochim Biophys Acta. 1963; 76:655-7. View

Keszenman-Pereyra D . Repair of UV-damaged incoming plasmid DNA in Saccharomyces cerevisiae. Photochem Photobiol. 1990; 51(3):331-42. DOI: 10.1111/j.1751-1097.1990.tb01719.x. View

Livneh Z . Mechanism of replication of ultraviolet-irradiated single-stranded DNA by DNA polymerase III holoenzyme of Escherichia coli. Implications for SOS mutagenesis. J Biol Chem. 1986; 261(20):9526-33. View

Messing J, Vieira J . A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982; 19(3):269-76. DOI: 10.1016/0378-1119(82)90016-6. View

Roberts R, Strike P . Efficiency of Escherichia coli repair processes on uv-damaged transforming plasmid DNA. Plasmid. 1981; 5(2):213-20. DOI: 10.1016/0147-619x(81)90022-6. View

TAKETO A, Yasuda S, Sekiguchi M . Initial step of excision repair in Escherichia coli: replacement of defective function of uvr mutants by T4 endonuclease V. J Mol Biol. 1972; 70(1):1-14. DOI: 10.1016/0022-2836(72)90160-x. View

10.

Kim R, Ray D . One strand of ars189 from the maxicircle of Crithidia fasciculata transforms Saccharomyces cerevisiae more efficiently than its complementary strand as a single stranded DNA. Gene. 1985; 40(2-3):285-90. DOI: 10.1016/0378-1119(85)90051-4. View

11.

SINSHEIMER R, STARMAN B, Nagler C, Guthrie S . The process of infection with bacteriophage phi-XI74. I. Evidence for a "replicative form". J Mol Biol. 1962; 4:142-60. DOI: 10.1016/s0022-2836(62)80047-3. View

12.

Strike P, Humphreys G, Roberts R . Nature of transforming deoxyribonucleic acid in calcium-treated Escherichia coli. J Bacteriol. 1979; 138(3):1033-5. PMC: 218138. DOI: 10.1128/jb.138.3.1033-1035.1979. View

13.

Rose M, Winston F . Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene. Mol Gen Genet. 1984; 193(3):557-60. DOI: 10.1007/BF00382100. View

14.

Friedberg E . Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae. Microbiol Rev. 1988; 52(1):70-102. PMC: 372706. DOI: 10.1128/mr.52.1.70-102.1988. View

15.

White C, Sedgwick S . The use of plasmid DNA to probe DNA repair functions in the yeast Saccharomyces cerevisiae. Mol Gen Genet. 1985; 201(1):99-106. DOI: 10.1007/BF00397993. View

16.

Silber J, ACHEY P . Excision repair participates in the Weigle reactivation of ultraviolet light-irradiated phi X174 double-stranded DNA. Mutat Res. 1984; 131(1):1-10. DOI: 10.1016/0167-8817(84)90041-5. View

17.

Yarus M, SINSHEIMER R . THE U.V.-RESISTANCE OF DOUBLE-STRANDED PHIX174 DNA. J Mol Biol. 1964; 8:614-5. DOI: 10.1016/s0022-2836(64)80018-8. View

18.

HANAWALT P, Cooper P, Ganesan A, Smith C . DNA repair in bacteria and mammalian cells. Annu Rev Biochem. 1979; 48:783-836. DOI: 10.1146/annurev.bi.48.070179.004031. View