Yeast Sec Proteins Interact with Polypeptides Traversing the Endoplasmic Reticulum Membrane

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 1992 Apr 17

PMID 1568249

Citations 47

Authors

A Musch

M Wiedmann

T A Rapoport

Affiliations

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Abstract

We show by photocross-linking that nascent secretory proteins, during their passage through the endoplasmic reticulum membrane of S. cerevisiae, are in physical contact with Sec61p and Sec62p, two genetically identified membrane proteins that are essential for in vivo translocation. Sec61p seems to be in continuous contact, whereas Sec62p is involved only transiently. Translocation comprises both ATP-dependent and -independent phases of interaction with the Sec proteins. The results suggest a direct role of the Sec proteins in translocation.

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