Lentiviral Transgenesis

Overview

Journal Transgenic Res

Specialty Molecular Biology

Date 2005 Jan 28

PMID 15672832

Citations 29

Authors

Alexander Pfeifer

Affiliations

Soon will be listed here.

Abstract

Transgenic animals are relevant for many fields of modern biomedicine and agriculture. However, the inefficiencies of the presently available techniques--DNA microinjection and retroviral gene transfer--have led to an explosion of costs for transgenics especially in farm animals. The recent success in transferring genes to early embryos of different species (mouse, rat, pig, cattle) by viral vectors derived from lentiviruses, has established lentiviral transgenesis as an exciting alternative to the classical method of DNA microinjection. In addition, lentiviral vectors can be used to transfer genes into embryonic stem cells. Due to its high efficacy and versatility, lentiviral transgenesis should have a big impact on transgenic research.

Citing Articles

The Gq signalling pathway inhibits brown and beige adipose tissue.

Klepac K, Kilic A, Gnad T, Brown L, Herrmann B, Wilderman A Nat Commun. 2016; 7:10895.

PMID: 26955961 PMC: 4786868. DOI: 10.1038/ncomms10895.

2015 Guidelines for Establishing Genetically Modified Rat Models for Cardiovascular Research.

Flister M, Prokop J, Lazar J, Shimoyama M, Dwinell M, Geurts A J Cardiovasc Transl Res. 2015; 8(4):269-77.

PMID: 25920443 PMC: 4475456. DOI: 10.1007/s12265-015-9626-4.

Gene transfer into cardiac myocytes.

Lang S, Westfall M Methods Mol Biol. 2015; 1299:177-90.

PMID: 25836585 PMC: 4741385. DOI: 10.1007/978-1-4939-2572-8_15.

Vector modifications to eliminate transposase expression following piggyBac-mediated transgenesis.

Chakraborty S, Ji H, Chen J, Gersbach C, Leong K Sci Rep. 2014; 4:7403.

PMID: 25492703 PMC: 4261183. DOI: 10.1038/srep07403.

Embryo development, fetal growth and postnatal phenotype of eGFP lambs generated by lentiviral transgenesis.

Crispo M, Vilarino M, Dos Santos-Neto P, Nunez-Olivera R, Cuadro F, Barrera N Transgenic Res. 2014; 24(1):31-41.

PMID: 25048992 DOI: 10.1007/s11248-014-9816-x.

References

Brinster R, Chen H, Trumbauer M, Yagle M, Palmiter R . Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc Natl Acad Sci U S A. 1985; 82(13):4438-42. PMC: 391116. DOI: 10.1073/pnas.82.13.4438. View

Hammer R, Pursel V, Rexroad Jr C, Wall R, Bolt D, Ebert K . Production of transgenic rabbits, sheep and pigs by microinjection. Nature. 1985; 315(6021):680-3. DOI: 10.1038/315680a0. View

Hochedlinger K, Jaenisch R . Nuclear transplantation, embryonic stem cells, and the potential for cell therapy. N Engl J Med. 2003; 349(3):275-86. DOI: 10.1056/NEJMra035397. View

Hamaguchi I, Woods N, Panagopoulos I, Andersson E, Mikkola H, Fahlman C . Lentivirus vector gene expression during ES cell-derived hematopoietic development in vitro. J Virol. 2000; 74(22):10778-84. PMC: 110952. DOI: 10.1128/jvi.74.22.10778-10784.2000. View

Wu X, Li Y, Crise B, Burgess S . Transcription start regions in the human genome are favored targets for MLV integration. Science. 2003; 300(5626):1749-51. DOI: 10.1126/science.1083413. View

Wolfgang M, Eisele S, Browne M, Schotzko M, Garthwaite M, Durning M . Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos. Proc Natl Acad Sci U S A. 2001; 98(19):10728-32. PMC: 58541. DOI: 10.1073/pnas.181336098. View

Lee N, Dohjima T, Bauer G, Li H, Li M, Ehsani A . Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat Biotechnol. 2002; 20(5):500-5. DOI: 10.1038/nbt0502-500. View

Gossen M, Bujard H . Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992; 89(12):5547-51. PMC: 49329. DOI: 10.1073/pnas.89.12.5547. View

Auerbach A, Norinsky R, Ho W, Losos K, Guo Q, Chatterjee S . Strain-dependent differences in the efficiency of transgenic mouse production. Transgenic Res. 2003; 12(1):59-69. DOI: 10.1023/a:1022166921766. View

10.

Nottle M, Haskard K, Verma P, Du Z, Grupen C, McIlfatrick S . Effect of DNA concentration on transgenesis rates in mice and pigs. Transgenic Res. 2002; 10(6):523-31. DOI: 10.1023/a:1013007329936. View

11.

Scherr M, Battmer K, Eder M, Schule S, Hohenberg H, Ganser A . Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography. Gene Ther. 2002; 9(24):1708-14. DOI: 10.1038/sj.gt.3301848. View

12.

Tiscornia G, Singer O, Ikawa M, Verma I . A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc Natl Acad Sci U S A. 2003; 100(4):1844-8. PMC: 149921. DOI: 10.1073/pnas.0437912100. View

13.

Pfeifer A, Verma I . Gene therapy: promises and problems. Annu Rev Genomics Hum Genet. 2001; 2:177-211. DOI: 10.1146/annurev.genom.2.1.177. View

14.

Wells K, Moore K, Wall R . Transgene vectors go retro. Nat Biotechnol. 1999; 17(1):25-6. DOI: 10.1038/5196. View

15.

Jaenisch R, Fan H, Croker B . Infection of preimplantation mouse embryos and of newborn mice with leukemia virus: tissue distribution of viral DNA and RNA and leukemogenesis in the adult animal. Proc Natl Acad Sci U S A. 1975; 72(10):4008-12. PMC: 433127. DOI: 10.1073/pnas.72.10.4008. View

16.

Rubinson D, Dillon C, Kwiatkowski A, Sievers C, Yang L, Kopinja J . A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet. 2003; 33(3):401-6. DOI: 10.1038/ng1117. View

17.

Schroder A, Shinn P, Chen H, Berry C, Ecker J, Bushman F . HIV-1 integration in the human genome favors active genes and local hotspots. Cell. 2002; 110(4):521-9. DOI: 10.1016/s0092-8674(02)00864-4. View

18.

Zufferey R, Donello J, Trono D, Hope T . Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J Virol. 1999; 73(4):2886-92. PMC: 104046. DOI: 10.1128/JVI.73.4.2886-2892.1999. View

19.

Zennou V, Petit C, Guetard D, Nerhbass U, Montagnier L, Charneau P . HIV-1 genome nuclear import is mediated by a central DNA flap. Cell. 2000; 101(2):173-85. DOI: 10.1016/S0092-8674(00)80828-4. View

20.

Whitelaw C . Transgenic livestock made easy. Trends Biotechnol. 2004; 22(4):157-9. DOI: 10.1016/j.tibtech.2004.02.005. View