Clustered Genes Required for the Synthesis of Heterocyst Envelope Polysaccharide in Anabaena Sp. Strain PCC 7120

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2005 Jan 22

PMID 15659688

Citations 28

Authors

Guocun Huang

Qing Fan

Sigal Lechno-Yossef

Elizabeth Wojciuch

C Peter Wolk

Takakazu Kaneko

Satoshi Tabata

Affiliations

Soon will be listed here.

Abstract

As demonstrated with alr2835 (hepA) and alr2834 (hepC) mutants, heterocysts of Anabaena sp. strain PCC 7120, a filamentous cyanobacterium, must have an envelope polysaccharide layer (the Hep+ phenotype) to fix dinitrogen in an oxygen-containing milieu (the Fox+ phenotype). Transpositions presumptively responsible for a Fox- phenotype were localized in open reading frames (ORFs) near hepA and hepC. A mutation in each of nine of these ORFs was complemented by a clone bearing only that single, intact ORF. Heterocysts of the nine mutants were found to lack an envelope polysaccharide layer. Complementation of mutations in alr2832 and alr2840 may have resulted from recombination. However, alr2825, alr2827, alr2831, alr2833, alr2837, alr2839, and alr2841, like hepA and hepC, are required for a Hep+ Fox+ phenotype.

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