A Multistep, GTP-driven Mechanism Controlling the Dynamic Cycling of Nucleostemin

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2005 Jan 20

PMID 15657390

Citations 92

Authors

Robert Y L Tsai

Ronald D G McKay

Affiliations

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Abstract

Nucleostemin (NS) was identified as a stem cell- and cancer cell-enriched nucleolar protein that controls the proliferation of these cells. Here, we report the mechanism that regulates its dynamic shuttling between the nucleolus and nucleoplasm. The nucleolar residence of nucleostemin involves a transient and a long-term binding by the basic and GTP-binding domains, and a dissociation mechanism mediated by the COOH-terminal region. This cycle is propelled by the GTP binding state of nucleostemin. We propose that a rapid nucleostemin cycle is designed to translate extra- and intra-cellular signals into the amount of nucleostemin in the nucleolus in a bidirectional and fast manner.

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