Large-scale DNA Microarray Analysis of Atopic Skin Lesions Shows Overexpression of an Epidermal Differentiation Gene Cluster in the Alternative Pathway and Lack of Protective Gene Expression in the Cornified Envelope

Overview

Journal Br J Dermatol

Publisher Oxford University Press

Specialty Dermatology

Date 2005 Jan 20

PMID 15656815

Citations 48

Authors

H Sugiura

H Ebise

T Tazawa

K Tanaka

Y Sugiura

M Uehara

K Kikuchi

T Kimura

Affiliations

Soon will be listed here.

Abstract

Background: Atopic dermatitis (AD)-specific genes have not yet been clarified. Objectives To identify gene expression specific to active atopic skin lesions.

Methods: We analysed 23,000 genes in skin biopsy samples from 17 patients with AD and four normal controls using Affymetrix oligonucleotide arrays.

Results: Four of the 10 genes with the greatest differences in expression between patients and controls, S100A8 and S100A7 (upregulated), and loricrin and filaggrin (downregulated), were epidermal differentiation genes located on 1q21, a locus previously reported to have a genetic linkage with AD.

Conclusions: Our results, showing downregulation of the cornified envelope genes and upregulation of the alternative keratinization pathway, are the first to suggest abnormal epidermal differentiation and defective defences as key abnormalities in AD.

Citing Articles

Increased intracellular stress responses and decreased KLF2 in adult patients with atopic dermatitis.

Sugiura S, Sugiura S, Yoshida H, Yoshida H, Sugiura H, Sugiura H Cell Stress Chaperones. 2025; 30(2):84-99.

PMID: 39938773 PMC: 11891603. DOI: 10.1016/j.cstres.2025.02.001.

The epidermal lipid-microbiome loop and immunity: Important players in atopic dermatitis.

Wu J, Li L, Zhang T, Lu J, Tai Z, Zhu Q J Adv Res. 2024; 68:359-374.

PMID: 38460775 PMC: 11785582. DOI: 10.1016/j.jare.2024.03.001.

Single-cell profiling of prurigo nodularis demonstrates immune-stromal crosstalk driving profibrotic responses and reversal with nemolizumab.

Ma F, Gharaee-Kermani M, Tsoi L, Plazyo O, Chaskar P, Harms P J Allergy Clin Immunol. 2023; 153(1):146-160.

PMID: 37506977 PMC: 11231883. DOI: 10.1016/j.jaci.2023.07.005.

Loricrin and Cytokeratin Disorganisation in Severe Forms of Periodontitis.

Catunda R, Ho K, Patel S, Roy C, Alexiou M, Levin L Int Dent J. 2023; 73(6):862-872.

PMID: 37316411 PMC: 10658443. DOI: 10.1016/j.identj.2023.05.004.

Genetic polymorphism (rs6587666) in protects from eczema in admixed Brazilian children population with high African ancestry.

Rios R, da Silva T, Strina A, Forno E, Costa R, Celedon J Heliyon. 2023; 9(2):e13659.

PMID: 36865480 PMC: 9970909. DOI: 10.1016/j.heliyon.2023.e13659.