Nitric Oxide Inhibits the Replication Cycle of Severe Acute Respiratory Syndrome Coronavirus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2005 Jan 15

PMID 15650225

Citations 184

Authors

Sara Akerstrom

Mehrdad Mousavi-Jazi

Jonas Klingstrom

Mikael Leijon

Ake Lundkvist

Ali Mirazimi

Affiliations

Soon will be listed here.

Abstract

Nitric oxide (NO) is an important signaling molecule between cells which has been shown to have an inhibitory effect on some virus infections. The purpose of this study was to examine whether NO inhibits the replication cycle of the severe acute respiratory syndrome coronavirus (SARS CoV) in vitro. We found that an organic NO donor, S-nitroso-N-acetylpenicillamine, significantly inhibited the replication cycle of SARS CoV in a concentration-dependent manner. We also show here that NO inhibits viral protein and RNA synthesis. Furthermore, we demonstrate that NO generated by inducible nitric oxide synthase, an enzyme that produces NO, inhibits the SARS CoV replication cycle.

Citing Articles

Herbal and Dietary Supplements as Adjunctive Treatment for Mild SARS-CoV-2 Infection in Italy.

Licata A, Seidita A, Como S, de Carlo G, Cammilleri M, Bonica R Nutrients. 2025; 17(2).

PMID: 39861359 PMC: 11767322. DOI: 10.3390/nu17020230.

Potential Beneficial Role of Nitric Oxide in SARS-CoV-2 Infection: Beyond Spike-Binding Inhibition.

Sanchez-Garcia S, Castrillo A, Bosca L, Prieto P Antioxidants (Basel). 2024; 13(11).

PMID: 39594443 PMC: 11591382. DOI: 10.3390/antiox13111301.

Impact of low dose inhaled nitric oxide treatment in spontaneously breathing and intubated COVID-19 patients: a retrospective propensity-matched study.

Isha S, Balasubramanian P, Hanson A, Jonna S, Raavi L, Khadka S Crit Care. 2024; 28(1):344.

PMID: 39456071 PMC: 11515277. DOI: 10.1186/s13054-024-05093-w.

Impact of Inhaled Nitric Oxide (iNO) on the Outcome of COVID-19 Associated ARDS.

Stoll S, Bottiger B, Dusse F, Leister N, Leupold T, Menzel C J Clin Med. 2024; 13(19).

PMID: 39408041 PMC: 11478273. DOI: 10.3390/jcm13195981.

A safety evaluation of intermittent high-dose inhaled nitric oxide in viral pneumonia due to COVID-19: a randomised clinical study.

Wolak T, Dicker D, Shifer Y, Grossman A, Rokach A, Shitrit M Sci Rep. 2024; 14(1):17201.

PMID: 39060420 PMC: 11282178. DOI: 10.1038/s41598-024-68055-w.

References

Akaike T, Noguchi Y, Ijiri S, Setoguchi K, Suga M, Zheng Y . Pathogenesis of influenza virus-induced pneumonia: involvement of both nitric oxide and oxygen radicals. Proc Natl Acad Sci U S A. 1996; 93(6):2448-53. PMC: 39817. DOI: 10.1073/pnas.93.6.2448. View

Akarid K, Sinet M, Desforges B, Gougerot-Pocidalo M . Inhibitory effect of nitric oxide on the replication of a murine retrovirus in vitro and in vivo. J Virol. 1995; 69(11):7001-5. PMC: 189619. DOI: 10.1128/JVI.69.11.7001-7005.1995. View

Lane T, Paoletti A, Buchmeier M . Disassociation between the in vitro and in vivo effects of nitric oxide on a neurotropic murine coronavirus. J Virol. 1997; 71(3):2202-10. PMC: 191327. DOI: 10.1128/JVI.71.3.2202-2210.1997. View

Adler H, Beland J, Kobzik L, Brewer J, Martin T, Rimm I . Suppression of herpes simplex virus type 1 (HSV-1)-induced pneumonia in mice by inhibition of inducible nitric oxide synthase (iNOS, NOS2). J Exp Med. 1997; 185(9):1533-40. PMC: 2196291. DOI: 10.1084/jem.185.9.1533. View

Lin Y, Huang Y, Ma S, Yeh C, Chiou S, Chen L . Inhibition of Japanese encephalitis virus infection by nitric oxide: antiviral effect of nitric oxide on RNA virus replication. J Virol. 1997; 71(7):5227-35. PMC: 191758. DOI: 10.1128/JVI.71.7.5227-5235.1997. View

Pope M, Marsden P, Cole E, Sloan S, Fung L, Ning Q . Resistance to murine hepatitis virus strain 3 is dependent on production of nitric oxide. J Virol. 1998; 72(9):7084-90. PMC: 109929. DOI: 10.1128/JVI.72.9.7084-7090.1998. View

Murphy A, Platts-Mills T, Lobo M, Hayden F . Respiratory nitric oxide levels in experimental human influenza. Chest. 1998; 114(2):452-6. DOI: 10.1378/chest.114.2.452. View

Karupiah G, Chen J, Mahalingam S, Nathan C, MacMicking J . Rapid interferon gamma-dependent clearance of influenza A virus and protection from consolidating pneumonitis in nitric oxide synthase 2-deficient mice. J Exp Med. 1998; 188(8):1541-6. PMC: 2213404. DOI: 10.1084/jem.188.8.1541. View

Boucher J, Moali C, Tenu J . Nitric oxide biosynthesis, nitric oxide synthase inhibitors and arginase competition for L-arginine utilization. Cell Mol Life Sci. 1999; 55(8-9):1015-28. PMC: 11147020. DOI: 10.1007/s000180050352. View

10.

Rimmelzwaan G, Baars M, de Lijster P, Fouchier R, Osterhaus A . Inhibition of influenza virus replication by nitric oxide. J Virol. 1999; 73(10):8880-3. PMC: 112914. DOI: 10.1128/JVI.73.10.8880-8883.1999. View

11.

Benencia F, Courreges M . Nitric oxide and macrophage antiviral extrinsic activity. Immunology. 1999; 98(3):363-70. PMC: 2326934. DOI: 10.1046/j.1365-2567.1999.00864.x. View

12.

Mirazimi A, Svensson L . ATP is required for correct folding and disulfide bond formation of rotavirus VP7. J Virol. 2000; 74(17):8048-52. PMC: 112337. DOI: 10.1128/jvi.74.17.8048-8052.2000. View

13.

Blond D, Raoul H, Le Grand R, Dormont D . Nitric oxide synthesis enhances human immunodeficiency virus replication in primary human macrophages. J Virol. 2000; 74(19):8904-12. PMC: 102085. DOI: 10.1128/jvi.74.19.8904-8912.2000. View

14.

Kao Y, Piedra P, Larsen G, Colasurdo G . Induction and regulation of nitric oxide synthase in airway epithelial cells by respiratory syncytial virus. Am J Respir Crit Care Med. 2001; 163(2):532-9. DOI: 10.1164/ajrccm.163.2.9912068. View

15.

Saxena S, Mathur A, Srivastava R . Induction of nitric oxide synthase during Japanese encephalitis virus infection: evidence of protective role. Arch Biochem Biophys. 2001; 391(1):1-7. DOI: 10.1006/abbi.2001.2360. View

16.

Coleman J . Nitric oxide in immunity and inflammation. Int Immunopharmacol. 2001; 1(8):1397-406. DOI: 10.1016/s1567-5769(01)00086-8. View

17.

Poljakovic M, Karpman D, Svanborg C, Persson K . Human renal epithelial cells express iNOS in response to cytokines but not bacteria. Kidney Int. 2002; 61(2):444-55. DOI: 10.1046/j.1523-1755.2002.00138.x. View

18.

Rota P, Oberste M, Monroe S, Nix W, Campagnoli R, Icenogle J . Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science. 2003; 300(5624):1394-9. DOI: 10.1126/science.1085952. View

19.

Marra M, Jones S, Astell C, Holt R, Brooks-Wilson A, Butterfield Y . The Genome sequence of the SARS-associated coronavirus. Science. 2003; 300(5624):1399-404. DOI: 10.1126/science.1085953. View

20.

Donnelly C, Ghani A, Leung G, Hedley A, Fraser C, Riley S . Epidemiological determinants of spread of causal agent of severe acute respiratory syndrome in Hong Kong. Lancet. 2003; 361(9371):1761-6. PMC: 7112380. DOI: 10.1016/S0140-6736(03)13410-1. View