Microsatellite Instability As Prognostic Marker in Bladder Tumors: a Clinical Significance

Overview

Journal BMC Urol

Publisher Biomed Central

Specialty Urology

Date 2005 Jan 14

PMID 15647110

Citations 13

Authors

Minal Vaish

Anil Mandhani

R D Mittal

Balraj Mittal

Affiliations

Soon will be listed here.

Abstract

Background: Carcinoma of urinary bladder is one of the leading causes of death in India. Successful treatment of bladder cancer depends on the early detection & specific diagnostic approaches. In the present study, microsatellite instability (MSI) has been evaluated as a prognostic marker in patients with superficial urinary bladder cancer in lower urinary tract for determining risk of recurrence.

Methods: A total of 44 patients with bladder tumors diagnosed with Transitional Cell Carcinomas [TCC] from lower urinary tract were selected for the study. Tumors were staged and graded according to AJCC-UICC (1997) classification and patients were followed with cystoscopy as per the protocol. Polymerase chain reaction (PCR) was done to amplify microsatellite sequences at mononucleotide BAT - 26, BAT - 40, TGFbeta RII, IGFIIR, hMSH3, BAX and dinucleotide D2S123, D9S283, D9S1851 and D18S58 loci in blood (control) and tumor DNA. PCR products were separated on 8% denaturing polyacrylamide gel and visualized by autoradiography.

Results: MSI was observed in 72.7% of tumors at BAT - 26, BAT - 40, D2S123, D9S283, D9S1851 and D18S58 loci. Good association of MSI was seen with tumor stage and grade. MSI - High (instability at > 30% of loci) was frequently observed in high stage (40.6%) and high grade (59.4%) tumors. Of 24 tumors of Ta-T1 stage with different grades, 11 (9/18 high grade and 2/6 low grade tumors) recurred in the mean duration of 36 months. MSI positivity was significantly high in patients who had one or more recurrences (p = 0.02 for high grade and 0.04 for low grade tumors).

Conclusions: MSI may be an independent prognostic marker for assessing risk of recurrence in superficial tumors irrespective of the grade. Further studies on progression would help in stratifying the patients of T1G3 for early cystectomy vs bladder preservation protocol.

Citing Articles

Microsatellite Instability in Urine: Breakthrough Method for Bladder Cancer Identification.

Rico-Mendez M, Ayala-Madrigal M, Gonzalez-Mercado A, Gutierrez-Angulo M, Ramirez de Arellano Sanchez J, Beltran-Ontiveros S Biomedicines. 2025; 12(12.

PMID: 39767633 PMC: 11727160. DOI: 10.3390/biomedicines12122726.

The Evolving Molecular Landscape and Actionable Alterations in Urologic Cancers.

Antar R, Fawaz C, Gonzalez D, Xu V, Drouaud A, Krastein J Curr Oncol. 2024; 31(11):6909-6937.

PMID: 39590142 PMC: 11593205. DOI: 10.3390/curroncol31110511.

Mismatch repair deficiency and microsatellite instability in urothelial carcinoma: a systematic review and meta-analysis.

Chandran E, Iannantuono G, Atiq S, Akbulut D, Sinaii N, Simon N BMJ Oncol. 2024; 3(1).

PMID: 39086924 PMC: 11203074. DOI: 10.1136/bmjonc-2024-000335.

Role of PD-1/PD-L1-mediated tumour immune escape mechanism and microsatellite instability in the BCG failure of high-grade urothelial carcinomas.

Salman F, Kankaya D, Ozakinci H, Sahin Y, Kubilay E, Suer E Turk J Med Sci. 2023; 52(6):1802-1813.

PMID: 36945968 PMC: 10390201. DOI: 10.55730/1300-0144.5526.

Utility of Contemporary Health Screening in the Diagnosis of Bladder Cancer.

Lee C, Song W, Koo M, Boo Y, Chung J, Kang M Diagnostics (Basel). 2022; 12(5).

PMID: 35626196 PMC: 9139924. DOI: 10.3390/diagnostics12051040.

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