Synthetic Trimer and Tetramer of 3-beta-D-ribose-(1-1)-D-ribitol-5-phosphate Conjugated to Protein Induce Antibody Responses to Haemophilus Influenzae Type B Capsular Polysaccharide in Mice and Monkeys

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1992 May 1

PMID 1563770

Citations 22

Authors

C C Peeters

D Evenberg

P Hoogerhout

H Kayhty

L Saarinen

C A van Boeckel

G A van der Marel

J H VAN Boom

J T Poolman

Affiliations

Soon will be listed here.

Abstract

Synthetic oligosaccharides derived from the capsular polysaccharide (PRP) of Haemophilus influenzae type b were conjugated to carrier proteins via a thioether linkage. Conjugates were made of trimeric and tetrameric ribose-ribitol-phosphate and tetanus toxoid or diphtheria toxin. All conjugates elicited anti-PRP antibody responses with an increasing immunoglobulin G/immunoglobulin M ratio in adult mice and monkeys. Trimer conjugates elicited lower anti-PRP antibody responses compared with tetramer conjugates. Adult monkeys responded equally well to the tetrameric oligosaccharide-tetanus toxoid conjugate as to the oligosaccharide-CRM197 conjugate (HbOC), which elicits protective levels of serum antibodies in human infants after two or three injections.

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