First Report of Vancomycin-resistant Staphylococci Isolated from Healthy Carriers in Brazil

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2005 Jan 7

PMID 15634969

Citations 27

Authors

I C V Palazzo

M L C Araujo

A L C Darini

Affiliations

Soon will be listed here.

Abstract

Reduced susceptibility or resistance to vancomycin has been reported among clinical isolates of staphylococci in previous studies. In the present study we report on the isolation of four vancomycin-resistant staphylococcal strains from healthy carriers inside and outside the hospital environment. These carriers did not receive treatment with any antibiotic. All coagulase-negative staphylococcal strains showed variable levels of resistance to several antimicrobial agents, including oxacillin, and unstable resistance to vancomycin, with decreased vancomycin MICs (<4 mg/liter) after 10 days of passage in a nonselective medium. However, exposure of these revertants to vancomycin selected staphylococcal strains resistant to vancomycin at very high frequencies (10(-2) and 10(-3)). The vancomycin resistance in these staphylococcal strains was not mediated by the van gene. The cell wall of the staphylococcal strains studied became thickest after culture in medium containing vancomycin, and the differences in cell wall thickness were statistically significant (P < 0.001). Thus, the thickening of the cell wall in these staphylococcal strains may be an important contributor to vancomycin resistance.

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References

Pina P, Marliere C, Vandenesch F, Bedos J, Etienne J, Allouch P . An outbreak of Staphylococcus aureus strains with reduced susceptibility to glycopeptides in a French general hospital. Clin Infect Dis. 2000; 31(5):1306-8. DOI: 10.1086/317464. View

Sieradzki K, Leski T, Dick J, Borio L, Tomasz A . Evolution of a vancomycin-intermediate Staphylococcus aureus strain in vivo: multiple changes in the antibiotic resistance phenotypes of a single lineage of methicillin-resistant S. aureus under the impact of antibiotics administered for chemotherapy. J Clin Microbiol. 2003; 41(4):1687-93. PMC: 153915. DOI: 10.1128/JCM.41.4.1687-1693.2003. View

Walker J, Borrow R, Edwards-Jones V, Oppenheim B, Fox A . Epidemiological characterization of methicillin-resistant Staphylococcus aureus isolated in the North West of England by protein A (spa) and coagulase (coa) gene polymorphisms. Epidemiol Infect. 1999; 121(3):507-14. PMC: 2809556. DOI: 10.1017/s0950268898001538. View

Evers S, Courvalin P . Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol. 1995; 33(1):24-7. PMC: 227872. DOI: 10.1128/jcm.33.1.24-27.1995. View

Kohner P, Uhl J, Kolbert C, Persing D, Cockerill 3rd F . Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp. J Clin Microbiol. 1999; 37(9):2952-61. PMC: 85421. DOI: 10.1128/JCM.37.9.2952-2961.1999. View

Livermore D . Antibiotic resistance in staphylococci. Int J Antimicrob Agents. 2001; 16 Suppl 1:S3-10. DOI: 10.1016/s0924-8579(00)00299-5. View

Jordens J . Genomic DNA digestion and ribotyping. Methods Mol Med. 2011; 15:17-31. DOI: 10.1385/0-89603-498-4:17. View

Watanakunakorn C . Mode of action and in-vitro activity of vancomycin. J Antimicrob Chemother. 1984; 14 Suppl D:7-18. DOI: 10.1093/jac/14.suppl_d.7. View

Hiramatsu K . Vancomycin-resistant Staphylococcus aureus: a new model of antibiotic resistance. Lancet Infect Dis. 2002; 1(3):147-55. DOI: 10.1016/S1473-3099(01)00091-3. View

10.

Tenover F, Weigel L, Appelbaum P, McDougal L, Chaitram J, McAllister S . Vancomycin-resistant Staphylococcus aureus isolate from a patient in Pennsylvania. Antimicrob Agents Chemother. 2003; 48(1):275-80. PMC: 310200. DOI: 10.1128/AAC.48.1.275-280.2004. View

11.

Berke S, Lee J, Daum R . Reversion of the glycopeptide resistance phenotype in Staphylococcus aureus clinical isolates. Antimicrob Agents Chemother. 2000; 44(2):272-7. PMC: 89670. DOI: 10.1128/AAC.44.2.272-277.2000. View

12.

Sieradzki K, Tomasz A . Inhibition of cell wall turnover and autolysis by vancomycin in a highly vancomycin-resistant mutant of Staphylococcus aureus. J Bacteriol. 1997; 179(8):2557-66. PMC: 179004. DOI: 10.1128/jb.179.8.2557-2566.1997. View

13.

Reverdy M, Nervi C, Rougier M, Bolmstrom A, Vandenesch F, Etienne J . Clinical isolate of vancomycin-heterointermediate Staphylococcus aureus susceptible to methicillin and in vitro selection of a vancomycin-resistant derivative. Antimicrob Agents Chemother. 2000; 45(1):349-52. PMC: 90291. DOI: 10.1128/AAC.45.1.349-352.2001. View

14.

Hussain F, Boyle-Vavra S, Shete P, Daum R . Evidence for a continuum of decreased vancomycin susceptibility in unselected Staphylococcus aureus clinical isolates. J Infect Dis. 2002; 186(5):661-7. DOI: 10.1086/342708. View

15.

Cui L, Murakami H, Kuwahara-Arai K, Hanaki H, Hiramatsu K . Contribution of a thickened cell wall and its glutamine nonamidated component to the vancomycin resistance expressed by Staphylococcus aureus Mu50. Antimicrob Agents Chemother. 2000; 44(9):2276-85. PMC: 90058. DOI: 10.1128/AAC.44.9.2276-2285.2000. View

16.

. Staphylococcus aureus resistant to vancomycin--United States, 2002. MMWR Morb Mortal Wkly Rep. 2002; 51(26):565-7. View

17.

Trakulsomboon S, Danchaivijitr S, Rongrungruang Y, Dhiraputra C, Susaemgrat W, Ito T . First report of methicillin-resistant Staphylococcus aureus with reduced susceptibility to vancomycin in Thailand. J Clin Microbiol. 2001; 39(2):591-5. PMC: 87781. DOI: 10.1128/JCM.39.2.591-595.2001. View

18.

Woodford N, Morrison D, Johnson A, Briant V, George R, Cookson B . Application of DNA probes for rRNA and vanA genes to investigation of a nosocomial cluster of vancomycin-resistant enterococci. J Clin Microbiol. 1993; 31(3):653-8. PMC: 262836. DOI: 10.1128/jcm.31.3.653-658.1993. View

19.

Goering R . Molecular epidemiology of nosocomial infection: analysis of chromosomal restriction fragment patterns by pulsed-field gel electrophoresis. Infect Control Hosp Epidemiol. 1993; 14(10):595-600. DOI: 10.1086/646645. View

20.

Reipert A, Ehlert K, Kast T, Bierbaum G . Morphological and genetic differences in two isogenic Staphylococcus aureus strains with decreased susceptibilities to vancomycin. Antimicrob Agents Chemother. 2003; 47(2):568-76. PMC: 151770. DOI: 10.1128/AAC.47.2.568-576.2003. View