Establishment and Characterization of a Human Large Cell Lung Cancer Cell Line with Neuroendocrine Differentiation

Overview

Journal Pathol Oncol Res

Specialty Oncology

Date 2004 Dec 28

PMID 15619644

Citations 2

Authors

Sedigheh Sharifzadeh

S Mohammad Owji

Abdul Mohammad Pezeshki

Zahra Malek-Hoseini

Perikala Vijayananda Kumar

Sied Mohammad Ali Ghayumi

Abbas Ghaderi

Affiliations

Soon will be listed here.

Abstract

Characterization of a human lung cancer cell line is reported. This cell line was established from a patient referred to Nemazi Hospital of Shiraz University of Medical Sciences with a diagnosis of poorly differentiated carcinoma. Sterile sample from peritoneal effusion was taken and immediately cultured in RPMI-1640 medium containing 20% FBS, at 37 degrees C with 5% CO2. This cell line has been in continuous culture for more than one year and has been named as Mehr-80. Several features of the cell line were investigated, including growth characteristics, electron microscopic features, cloning efficiency in soft agar, expression of various antigenic markers, chromosomal and DNA analysis. On the basis of morphological and immunohistochemical analysis of Mehr-80, it is possible to conclude that this cell line is characterized by features similar to those reported for large cell carcinoma with neuroendocrine differentiation (LCCND). This cell line will be a valuable in vitro tool for further studies on lung cancers.

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References

Shibuya T . Establishment of a G-CSF and GM-CSF producing cell line from human large cell carcinoma of the lung. Eur J Haematol. 1989; 43(2):182-3. DOI: 10.1111/j.1600-0609.1989.tb00276.x. View

Jiang S, Kameya T, Shoji M, Dobashi Y, Shinada J, Yoshimura H . Large cell neuroendocrine carcinoma of the lung: a histologic and immunohistochemical study of 22 cases. Am J Surg Pathol. 1998; 22(5):526-37. DOI: 10.1097/00000478-199805000-00002. View

Bonner A, Lemon W, Devereux T, Lubet R, You M . Molecular profiling of mouse lung tumors: association with tumor progression, lung development, and human lung adenocarcinomas. Oncogene. 2003; 23(5):1166-76. DOI: 10.1038/sj.onc.1207234. View

Pisani P, Parkin D, Bray F, Ferlay J . Estimates of the worldwide mortality from 25 cancers in 1990. Int J Cancer. 1999; 83(1):18-29. DOI: 10.1002/(sici)1097-0215(19990924)83:1<18::aid-ijc5>3.0.co;2-m. View

Yousem S, Taylor S . Typical and atypical carcinoid tumors of lung: a clinicopathologic and DNA analysis of 20 tumors. Mod Pathol. 1990; 3(4):502-7. View

Volm M, Mattern J, SONKA J, Vogt-Schaden M, Wayss K . DNA distribution in non-small-cell lung carcinomas and its relationship to clinical behavior. Cytometry. 1985; 6(4):348-56. DOI: 10.1002/cyto.990060412. View

Yamagata N, Shyr Y, Yanagisawa K, Edgerton M, Dang T, Gonzalez A . A training-testing approach to the molecular classification of resected non-small cell lung cancer. Clin Cancer Res. 2003; 9(13):4695-704. View

Difilippantonio S, Chen Y, Pietas A, Schluns K, Pacyna-Gengelbach M, Deutschmann N . Gene expression profiles in human non-small and small-cell lung cancers. Eur J Cancer. 2003; 39(13):1936-47. DOI: 10.1016/s0959-8049(03)00419-2. View

Lim E, Aggarwal A, Agasthian T, Wong P, Tan C, Sim E . Feasibility of using low-volume tissue samples for gene expression profiling of advanced non-small cell lung cancers. Clin Cancer Res. 2003; 9(16 Pt 1):5980-7. View

10.

Kozaki K, Miyaishi O, Tsukamoto T, Tatematsu Y, Hida T, Takahashi T . Establishment and characterization of a human lung cancer cell line NCI-H460-LNM35 with consistent lymphogenous metastasis via both subcutaneous and orthotopic propagation. Cancer Res. 2000; 60(9):2535-40. View

11.

Visscher D, Zarbo R, Trojanowski J, Sakr W, Crissman J . Neuroendocrine differentiation in poorly differentiated lung carcinomas: a light microscopic and immunohistologic study. Mod Pathol. 1990; 3(4):508-12. View

12.

Upton M, Hirohashi S, Tome Y, Miyazawa N, Suemasu K, Shimosato Y . Expression of vimentin in surgically resected adenocarcinomas and large cell carcinomas of lung. Am J Surg Pathol. 1986; 10(8):560-7. DOI: 10.1097/00000478-198608000-00006. View

13.

ROBERTS H, Komaki R, Allen P, El-Naggar A . Prognostic significance of DNA content in stage I adenocarcinoma of the lung. Int J Radiat Oncol Biol Phys. 1998; 41(3):573-8. DOI: 10.1016/s0360-3016(98)00099-6. View

14.

Suzuki A, Takahashi T, Okuno Y, Nakamura K, Tashiro H, Fukumoto M . Analysis of abnormal expression of g-csf gene in a novel tumor cell line (KHC 287) elaborating G-CSF, IL-1 and IL-6 with co-amplification of c-myc and c-ki-ras. Int J Cancer. 1991; 48(3):428-33. DOI: 10.1002/ijc.2910480321. View

15.

Iyoda A, Hiroshima K, Baba M, Saitoh Y, Ohwada H, Fujisawa T . Pulmonary large cell carcinomas with neuroendocrine features are high-grade neuroendocrine tumors. Ann Thorac Surg. 2002; 73(4):1049-54. DOI: 10.1016/s0003-4975(01)03616-5. View

16.

Sekido Y, Sato M, Usami N, Shigemitsu K, Mori S, Maeda O . Establishment of a large cell lung cancer cell line (Y-ML-1B) producing granulocyte colony-stimulating factor. Cancer Genet Cytogenet. 2002; 137(1):33-42. DOI: 10.1016/s0165-4608(02)00536-8. View

17.

Macikova I, Perzelova A, Mraz P, Steno J, Bizik I . Establishment, morphological, growth and cytoskeletal properties of 135-BCA carcinoma cell line derived from lung brain metastasis. Neoplasma. 2002; 48(6):479-82. View

18.

Shijubo N, Inoue Y, Hirasawa M, Igarashi T, Mori M, Matsuura A . Granulocyte colony-stimulating factor-producing large cell undifferentiated carcinoma of the lung. Intern Med. 1992; 31(2):277-80. DOI: 10.2169/internalmedicine.31.277. View

19.

Harada R, Uemura Y, Kobayashi M, Zamecnikova A, Nakata H, Taguchi T . Establishment and characterization of a new lung cancer cell line (MI-4) producing high levels of granulocyte colony stimulating factor. Jpn J Cancer Res. 2002; 93(6):667-76. PMC: 5927050. DOI: 10.1111/j.1349-7006.2002.tb01305.x. View

20.

YESNER R . Heterogeneity of so-called neuroendocrine lung tumors. Exp Mol Pathol. 2001; 70(3):179-82. DOI: 10.1006/exmp.2001.2373. View