Splicing of U12-type Introns Deposits an Exon Junction Complex Competent to Induce Nonsense-mediated MRNA Decay

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2004 Dec 21

PMID 15608055

Citations 13

Authors

Tetsuro Hirose

Mei-Di Shu

Joan A Steitz

Affiliations

Soon will be listed here.

Abstract

Metazoan cells have two pathways for intron removal involving the U2- and U12-type spliceosomes, which contain mostly nonoverlapping sets of small nuclear ribonucleoproteins. We show that in vitro splicing of a U12-type intron assembles an exon junction complex (EJC) that is comparably positioned and contains many of the same components as that deposited by the U2-type spliceosome. The presence of a U12-type intron downstream of a premature termination codon within an open reading frame (ORF) induces nonsense-mediated decay of the mRNA in vivo. These findings suggest a common pathway for EJC assembly by the two spliceosomes and highlight the evolutionary age of the EJC and its downstream functions in gene expression.

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