Detection of Gastrin and Its Messenger RNA in Zollinger-Ellison Tumors by Non-radioactive in Situ Hybridization and Immunocytochemistry

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1992 Jan 1

PMID 1559841

Citations 5

Authors

L I Larsson

D M Hougaard

Affiliations

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Abstract

Gastrin immunocytochemistry and non-radioactive in situ hybridization, using biotinylated oligonucleotide probes, for gastrin mRNA have been used for studying a retrospective material of six gastrin-producing (Zollinger-Ellison) tumors. Hybridization results for gastrin mRNA were positive in all six, while gastrin immunoreactivity could be detected in five tumors. In one of the patients, different areas of the same tumor displayed differences in immunoreactivity to gastrin, but were uniformly hybridization positive. Weak hybridization signals were detected in liver metastases from a necropsy case, while the gastrin immunostaining was more pronounced. The results show that non-radioactive hybridization methods are applicable to routine clinical specimens stored for as long as 16 years and that in situ hybridization may be a useful complement to immunocytochemical diagnosis, particularly in cases where high synthesis and little storage of hormonal products occur.

Citing Articles

The LIM-homeodomain protein Isl-1 segregates with somatostatin but not with gastrin expression during differentiation of somatostatin/gastrin precursor cells.

Larsson L, Tingstedt J, Madsen O, Serup P, Hougaard D Endocrine. 2010; 3(7):519-24.

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Coexpression of the gastrin and somatostatin genes in differentiating and neoplastic human cells.

Larsson L, Tingstedt J, Hougaard D Histochem Cell Biol. 1995; 104(2):139-44.

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Prevalence of erm gene classes in erythromycin-resistant Staphylococcus aureus strains isolated between 1959 and 1988.

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Adaptation of a non-radioactive in situ hybridization method to electron microscopy: detection of tenascin mRNAs in mouse cerebellum with digoxigenin-labelled probes and gold-labelled antibodies.

Dorries U, Bartsch U, Nolte C, Roth J, Schachner M Histochemistry. 1993; 99(3):251-62.

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Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridization. Expression of mRNA of the seminal vesicle secretion protein II and androgen receptor genes in the rat prostate.

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