Xenon Prevents Cellular Damage in Differentiated PC-12 Cells Exposed to Hypoxia

Overview

Journal BMC Neurosci

Publisher Biomed Central

Specialty Neurology

Date 2004 Dec 14

PMID 15588278

Citations 9

Authors

Christian Petzelt

Per Blom

Wolfgang Schmehl

Jana Muller

Wolfgang J Kox

Affiliations

Soon will be listed here.

Abstract

Background: The neuroprotective effect of xenon has been demonstrated for glutamatergic neurons. In the present study it is investigated if dopaminergic neurons, i.e. nerve-growth-factor differentiated PC-12 cells, are protected as well against hypoxia-induced cell damage in the presence of xenon.

Results: Pheochromocytoma cells differentiated by addition of nerve growth factor were placed in a N2-saturated atmosphere, a treatment that induced release of dopamine, reaching a maximum after 30 min. By determining extracellular lactate dehydrogenase concentration as marker for concomitant cellular damage, a substantial increase of enzymatic activity was found for N2-treated cells. Replacement of N2 by xenon in such a hypoxic atmosphere resulted in complete protection against cellular damage and prevention of hypoxia-induced dopamine release. Intracellular buffering of Ca2+ using the Ca-chelator 1, 2-bis(2-Aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester (BAPTA) reduced the neuroprotective effect of xenon indicating the essential participation of intracellular Ca2+-ions in the process of xenon-induced neuroprotection.

Conclusions: The results presented demonstrate the outstanding property of xenon to protect neuron-like cells in a hypoxic situation.

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