The F-ATPase Operon Promoter of Streptococcus Mutans is Transcriptionally Regulated in Response to External PH

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2004 Dec 4

PMID 15576803

Citations 52

Authors

Wendi L Kuhnert

Guolu Zheng

Roberta C Faustoferri

Robert G Quivey Jr

Affiliations

Soon will be listed here.

Abstract

Streptococcus mutans F-ATPase, the major component of the acid-adaptive response of the organism, is transcriptionally upregulated at low pH. Fusions of the F-ATPase promoter to chloramphenicol acetyltransferase indicated that pH-dependent expression is still observed with a short promoter that contains a domain conserved between streptococcal ATPase operons.

Citing Articles

Environment signal dependent biocontainment systems for engineered organisms: Leveraging triggered responses and combinatorial systems.

Varma S, Gulati K, Sriramakrishnan J, Ganla R, Raval R Synth Syst Biotechnol. 2025; 10(2):356-364.

PMID: 39830078 PMC: 11741035. DOI: 10.1016/j.synbio.2024.12.005.

Genomic analysis of acid tolerance genes and deciphering the function of gene in mitigating acid tolerance in .

Sharma D, Chetri P, Ranga V, Sen S, Sarmah B, Barooah M Front Microbiol. 2024; 15:1414777.

PMID: 38966390 PMC: 11222612. DOI: 10.3389/fmicb.2024.1414777.

Contemporary strategies and approaches for characterizing composition and enhancing biofilm penetration targeting bacterial extracellular polymeric substances.

Lu L, Zhao Y, Li M, Wang X, Zhu J, Liao L J Pharm Anal. 2024; 14(4):100906.

PMID: 38634060 PMC: 11022105. DOI: 10.1016/j.jpha.2023.11.013.

Acid-tolerant bacteria and prospects in industrial and environmental applications.

Mallick S, Das S Appl Microbiol Biotechnol. 2023; 107(11):3355-3374.

PMID: 37093306 DOI: 10.1007/s00253-023-12529-w.

Effect of Extracts, Fractions, and Isolated Molecules of to Control Cariogenic Biofilm.

Ribeiro S, Bueno P, Cavalheiro A, Klein M Antibiotics (Basel). 2023; 12(2).

PMID: 36830240 PMC: 9952592. DOI: 10.3390/antibiotics12020329.

References

Belli W, Marquis R . Adaptation of Streptococcus mutans and Enterococcus hirae to acid stress in continuous culture. Appl Environ Microbiol. 1991; 57(4):1134-8. PMC: 182857. DOI: 10.1128/aem.57.4.1134-1138.1991. View

Wen Z, Burne R . Construction of a new integration vector for use in Streptococcus mutans. Plasmid. 2001; 45(1):31-6. DOI: 10.1006/plas.2000.1498. View

Gibbons R, Berman K, Knoettner P, KAPSIMALIS B . Dental caries and alveolar bone loss in gnotobiotic rats infected with capsule forming streptococci of human origin. Arch Oral Biol. 1966; 11(6):549-60. DOI: 10.1016/0003-9969(66)90220-2. View

Hamilton I, Buckley N . Adaptation by Streptococcus mutans to acid tolerance. Oral Microbiol Immunol. 1991; 6(2):65-71. DOI: 10.1111/j.1399-302x.1991.tb00453.x. View

Bailey T, Elkan C . Fitting a mixture model by expectation maximization to discover motifs in biopolymers. Proc Int Conf Intell Syst Mol Biol. 1994; 2:28-36. View

Quivey Jr R, Kuhnert W, Hahn K . Adaptation of oral streptococci to low pH. Adv Microb Physiol. 2000; 42:239-74. DOI: 10.1016/s0065-2911(00)42004-7. View

Dashper S, Reynolds E . pH regulation by Streptococcus mutans. J Dent Res. 1992; 71(5):1159-65. DOI: 10.1177/00220345920710050601. View

Ajdic D, McShan W, McLaughlin R, Savic G, Chang J, Carson M . Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc Natl Acad Sci U S A. 2002; 99(22):14434-9. PMC: 137901. DOI: 10.1073/pnas.172501299. View

Kobayashi H . A proton-translocating ATPase regulates pH of the bacterial cytoplasm. J Biol Chem. 1985; 260(1):72-6. View

10.

Bender G, Sutton S, Marquis R . Acid tolerance, proton permeabilities, and membrane ATPases of oral streptococci. Infect Immun. 1986; 53(2):331-8. PMC: 260879. DOI: 10.1128/iai.53.2.331-338.1986. View

11.

Kobayashi H, Suzuki T, UNEMOTO T . Streptococcal cytoplasmic pH is regulated by changes in amount and activity of a proton-translocating ATPase. J Biol Chem. 1986; 261(2):627-30. View

12.

Quivey Jr R, Faustoferri R, Belli W, Flores J . Polymerase chain reaction amplification, cloning, sequence determination and homologies of streptococcal ATPase-encoding DNAs. Gene. 1991; 97(1):63-8. DOI: 10.1016/0378-1119(91)90010-9. View

13.

Bailey T, Gribskov M . Methods and statistics for combining motif match scores. J Comput Biol. 1998; 5(2):211-21. DOI: 10.1089/cmb.1998.5.211. View

14.

Leblanc D, Lee L . Molecular, genetic, and functional analysis of the basic replicon of pVA380-1, a plasmid of oral streptococcal origin. Plasmid. 1992; 28(2):130-45. DOI: 10.1016/0147-619x(92)90044-b. View

15.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

16.

McDermid A, McKee A, Ellwood D, Marsh P . The effect of lowering the pH on the composition and metabolism of a community of nine oral bacteria grown in a chemostat. J Gen Microbiol. 1986; 132(5):1205-14. DOI: 10.1099/00221287-132-5-1205. View

17.

Ma Y, CURRAN T, Marquis R . Rapid procedure for acid adaptation of oral lactic-acid bacteria and further characterization of the response. Can J Microbiol. 1997; 43(2):143-8. DOI: 10.1139/m97-019. View

18.

Slots J, Ashimoto A, Flynn M, Li G, Chen C . Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction. Clin Infect Dis. 1995; 20 Suppl 2:S304-7. DOI: 10.1093/clinids/20.supplement_2.s304. View

19.

Sturr M, Marquis R . Comparative acid tolerances and inhibitor sensitivities of isolated F-ATPases of oral lactic acid bacteria. Appl Environ Microbiol. 1992; 58(7):2287-91. PMC: 195769. DOI: 10.1128/aem.58.7.2287-2291.1992. View

20.

Zheng M, Wang X, Doan B, Lewis K, Schneider T, Storz G . Computation-directed identification of OxyR DNA binding sites in Escherichia coli. J Bacteriol. 2001; 183(15):4571-9. PMC: 95352. DOI: 10.1128/JB.183.15.4571-4579.2001. View