Evidence for Involvement of at Least Six Proteins in Adaptation of Lactobacillus Sakei to Cold Temperatures and Addition of NaCl

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2004 Dec 3

PMID 15574925

Citations 19

Authors

Anika Marceau

Monique Zagorec

Stephane Chaillou

Therese Mera

Marie-Christine Champomier-Verges

Affiliations

Soon will be listed here.

Abstract

Lactobacillus sakei is a lactic acid bacterium widely represented in the natural flora of fresh meat. The aim of this study was to analyze the differences in protein expression during environmental changes encountered during technological processes in which L. sakei is involved in order to gain insight into the ability of this species to grow and survive in such environments. Using two-dimensional electrophoresis, we observed significant variation of a set of 21 proteins in cells grown at 4 degrees C or in the presence of 4% NaCl. Six proteins could be identified by determination of their N-terminal sequences, and the corresponding gene clusters were studied. Two proteins belong to carbon metabolic pathways, and four can be clustered as general stress proteins. A phenotype was observed at low temperature for five of the six mutants constructed for these genes. The survival of four mutants during stationary phase at 4 degrees C was affected, and surprisingly, one mutant showed enhanced survival during stationary phase at low temperatures.

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