Cyclophosphamide Affects the Dynamics of Granuloma Formation in Experimental Visceral Leishmaniasis

Overview

Journal Parasitol Res

Specialty Parasitology

Date 1992 Jan 1

PMID 1557327

Citations 4

Authors

E B Correa

J M Cunha

E D Madeira

Affiliations

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Abstract

We observed histopathological and ultrastructural hepatic changes following the intracardiac inoculation of Leishmania donovani amastigotes into inbred LHC hamsters (group I). Since granuloma formation is known to be T-cell-dependent, we also examined infected hamsters under cyclophosphamide immunosuppressive treatment (group ICy) and evaluated the production of interleukin-2 (IL-2) by their cells. Group I showed more intense hepatocyte and endothelial cell clasmatosis as well as hepatocyte degeneration and necrosis, deposits of connective tissue fibers, granulomas with multinucleated giant cells (MGCs) of foreign-body and Langhans' types and reduced production of IL-2 by spleen cells. In contrast, group ICy hamsters exhibited larger eosinophil and lymphocyte populations within sinusoids and peri-sinusoidal areas but showed no MGCs in granulomas. A striking decline in IL-2 production was noted. These results suggest that cyclophosphamide induces a delay in the natural evolution of L. donovani-induced granulomatous hepatic inflammation.

Citing Articles

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TGF-beta and mesenchymal hepatic involvement after visceral leishmaniasis.

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Tissue granuloma structure-function in experimental visceral leishmaniasis.

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