Definition of Sequence Requirements for Latency-associated Nuclear Antigen 1 Binding to Kaposi's Sarcoma-associated Herpesvirus DNA

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2004 Nov 27

PMID 15564510

Citations 24

Authors

Viswanathan Srinivasan

Takashi Komatsu

Mary E Ballestas

Kenneth M Kaye

Affiliations

Soon will be listed here.

Abstract

In latent infection, Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen 1 (LANA1)-specific binding to KSHV terminal repeat DNA mediates multicopy episome persistence. We now use electrophoretic mobility shift assays to investigate LANA1 binding to its 20-bp cognate sequence. Mutations at positions 6, 7, and 8 ((6)CCC(8)) severely reduced LANA1 binding, whereas mutations at other positions only modestly reduced binding. Since (6)CCC(8) is in the 5' half of an inverted repeat sequence, these results are consistent with an asymmetric role for the inverted repeat in LANA1 binding.

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