Protein Oligomerization Modulates Raft Partitioning and Apical Sorting of GPI-anchored Proteins

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2004 Nov 24

PMID 15557121

Citations 93

Authors

Simona Paladino

Daniela Sarnataro

Rudolf Pillich

Simona Tivodar

Lucio Nitsch

Chiara Zurzolo

Affiliations

Soon will be listed here.

Abstract

An essential but insufficient step for apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) in epithelial cells is their association with detergent-resistant microdomains (DRMs) or rafts. In this paper, we show that in MDCK cells both apical and basolateral GPI-APs associate with DRMs during their biosynthesis. However, only apical and not basolateral GPI-APs are able to oligomerize into high molecular weight complexes. Protein oligomerization begins in the medial Golgi, concomitantly with DRM association, and is dependent on protein-protein interactions. Impairment of oligomerization leads to protein missorting. We propose that oligomerization stabilizes GPI-APs into rafts and that this additional step is required for apical sorting of GPI-APs. Two alternative apical sorting models are presented.

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