Production and Characterisation of Cell- and Tissue-specific Monoclonal Antibodies for the Flatworm Macrostomum Sp

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 2004 Nov 20

PMID 15551153

Citations 22

Authors

Peter Ladurner

Daniela Pfister

Christof Seifarth

Lukas Scharer

Monika Mahlknecht

Willi Salvenmoser

Regine Gerth

Florentine Marx

Reinhard Rieger

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibodies (mABs) against various cell types of the basal free-living flatworm Macrostomum sp. were produced by immunising Balb/c mice with cell suspensions of disintegrated animals. We identified 360 positive supernatants with specific staining of various tissues, cell types, patterns or structures. Here we report immunocytochemical characterisation, histological stainings and isotyping of 11 mABs specific for muscle cells (MMu-1, MMu-2, MMu-3, MMu-4), digestive and prostate glands (MDr-1 and MDr-2, MPr-1), epidermal cells (MEp-1), the ventral nerve cord including neuron clusters (MNv-1), gastrodermal cells (MDa-1) and spermatids (MSp-1). Confocal microscopy, histological techniques, electron microscopy and immunoblotting were applied to demonstrate stainings in juveniles, adults, starved or well-fed animals. Considering the current lack of specific markers the obtained mABs will be particularly helpful studying embryonic and postembryonic development, pattern formation, cell differentiation, regeneration and reproductive allocation in Macrostomum sp., and possibly other basal flatworms. The small size, ease of culturing, short generation time, transparency and the basal phylogenetic position specify Macrostomum sp. as a suitable model organism for comparative analyses within Platyhelminthes and to Drosophila and C. elegans.

Citing Articles

Gene Is Required for Spermatogenesis in the Flatworm .

Biryukov M, Dmitrieva A, Vavilova V, Ustyantsev K, Bazarova E, Sukhikh I Int J Mol Sci. 2022; 23(23).

PMID: 36499445 PMC: 9740662. DOI: 10.3390/ijms232315110.

The free-living flatworm .

Wudarski J, Egger B, Ramm S, Scharer L, Ladurner P, Zadesenets K Evodevo. 2020; 11:5.

PMID: 32158530 PMC: 7053086. DOI: 10.1186/s13227-020-00150-1.

Genetic and environmental variation in transcriptional expression of seminal fluid proteins.

Patlar B, Weber M, Ramm S Heredity (Edinb). 2018; 122(5):595-611.

PMID: 30356222 PMC: 6461930. DOI: 10.1038/s41437-018-0160-4.

A targeted in situ hybridization screen identifies putative seminal fluid proteins in a simultaneously hermaphroditic flatworm.

Weber M, Wunderer J, Lengerer B, Pjeta R, Rodrigues M, Scharer L BMC Evol Biol. 2018; 18(1):81.

PMID: 29848299 PMC: 5977470. DOI: 10.1186/s12862-018-1187-0.

Transcriptional signatures of somatic neoblasts and germline cells in .

Grudniewska M, Mouton S, Simanov D, Beltman F, Grelling M, De Mulder K Elife. 2016; 5.

PMID: 27997336 PMC: 5173321. DOI: 10.7554/eLife.20607.

References

Reiter D, Ladurner P, Mair G, Salvenmoser W, Rieger R, Boyer B . Differentiation of the body wall musculature in Macrostomum hystricinum marinum and Hoploplana inquilina (Plathelminthes), as models for muscle development in lower Spiralia. Rouxs Arch Dev Biol. 2017; 205(7-8):410-423. DOI: 10.1007/BF00377221. View

Kubota Y, Morita T, Ito K . New monoclonal antibody (4E9R) identifies mouse neural crest cells. Dev Dyn. 1996; 206(4):368-78. DOI: 10.1002/(SICI)1097-0177(199608)206:4<368::AID-AJA3>3.0.CO;2-G. View

Dunne J, Javois L, Huang L, Bode H . A subset of cells in the nerve net of Hydra oligactis defined by a monoclonal antibody: its arrangement and development. Dev Biol. 1985; 109(1):41-53. DOI: 10.1016/0012-1606(85)90344-6. View

Crandall I, Newell P . Changes in cell surface glycoproteins during Dictyostelium development analysed using monoclonal antibodies. Development. 1989; 107(1):87-94. DOI: 10.1242/dev.107.1.87. View

Kobayashi T, Kajiura-Kobayashi H, Nagahama Y . Differential expression of vasa homologue gene in the germ cells during oogenesis and spermatogenesis in a teleost fish, tilapia, Oreochromis niloticus. Mech Dev. 2000; 99(1-2):139-42. DOI: 10.1016/s0925-4773(00)00464-0. View

Ladurner P, Rieger R, Baguna J . Spatial distribution and differentiation potential of stem cells in hatchlings and adults in the marine platyhelminth macrostomum sp.: a bromodeoxyuridine analysis. Dev Biol. 2000; 226(2):231-41. DOI: 10.1006/dbio.2000.9867. View

Goetinck S, Waterston R . The Caenorhabditis elegans UNC-87 protein is essential for maintenance, but not assembly, of bodywall muscle. J Cell Biol. 1994; 127(1):71-8. PMC: 2120180. DOI: 10.1083/jcb.127.1.71. View

Henry J, Martindale M, Boyer B . The unique developmental program of the acoel flatworm, Neochildia fusca. Dev Biol. 2001; 220(2):285-95. DOI: 10.1006/dbio.2000.9628. View

Agata K, Tanaka T, Kobayashi C, Kato K, Saitoh Y . Intercalary regeneration in planarians. Dev Dyn. 2003; 226(2):308-16. DOI: 10.1002/dvdy.10249. View

10.

Nimeth K, Mahlknecht M, Mezzanato A, Peter R, Rieger R, Ladurner P . Stem cell dynamics during growth, feeding, and starvation in the basal flatworm Macrostomum sp. (Platyhelminthes). Dev Dyn. 2004; 230(1):91-9. DOI: 10.1002/dvdy.20035. View

11.

Saumweber H . The use of monoclonal antibody libraries. Methods Cell Biol. 1991; 35:229-52. DOI: 10.1016/s0091-679x(08)60575-0. View

12.

Orii H, Ito H, Watanabe K . Anatomy of the planarian Dugesia japonica I. The muscular system revealed by antisera against myosin heavy chains. Zoolog Sci. 2002; 19(10):1123-31. DOI: 10.2108/zsj.19.1123. View

13.

Javois L . Patterning of the head in hydra as visualized by a monoclonal antibody: III. The dynamics of head regeneration. J Exp Zool. 1990; 254(2):155-64. DOI: 10.1002/jez.1402540207. View

14.

Javois L, Wood R, Bode H . Patterning of the head in hydra as visualized by a monoclonal antibody. I. Budding and regeneration. Dev Biol. 1986; 117(2):607-18. DOI: 10.1016/0012-1606(86)90329-5. View

15.

Mair G, Maule A, Day T, Halton D . A confocal microscopical study of the musculature of adult Schistosoma mansoni. Parasitology. 2000; 121 ( Pt 2):163-70. DOI: 10.1017/s0031182099006174. View

16.

Sanchez Alvarado A, Newmark P . The use of planarians to dissect the molecular basis of metazoan regeneration. Wound Repair Regen. 1998; 6(4):413-20. DOI: 10.1046/j.1524-475x.1998.60418.x. View

17.

Rieger R, Ladurner P . The significance of muscle cells for the origin of mesoderm in bilateria. Integr Comp Biol. 2011; 43(1):47-54. DOI: 10.1093/icb/43.1.47. View

18.

Yaross M, Westerfield J, Javois L, Bode H . Nerve cells in hydra: monoclonal antibodies identify two lineages with distinct mechanisms for their incorporation into head tissue. Dev Biol. 1986; 114(1):225-37. DOI: 10.1016/0012-1606(86)90398-2. View

19.

Gschwentner R, Ladurner P, Nimeth K, Rieger R . Stem cells in a basal bilaterian. S-phase and mitotic cells in Convolutriloba longifissura (Acoela, Platyhelminthes). Cell Tissue Res. 2001; 304(3):401-8. DOI: 10.1007/s004410100375. View

20.

Agata K . The Zoological Society Prize. Molecular and cellular approaches to planarian regeneration. Zoolog Sci. 2003; 19(12):1391-2. DOI: 10.2108/zsj.19.1391. View