Identification and Characterization of a QTL on Chromosome 2 for Cytosolic Glutamine Synthetase Content and Panicle Number in Rice

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2004 Nov 19

PMID 15549232

Citations 34

Authors

Mitsuhiro Obara

Tadashi Sato

Shohei Sasaki

Kenji Kashiba

Atsushi Nagano

Ikuo Nakamura

Takeshi Ebitani

Masahiro Yano

Tomoyuki Yamaya

Affiliations

Soon will be listed here.

Abstract

A quantitative trait locus (QTL) associated with the protein content of cytosolic glutamine synthetase (GS1; EC 6.3.1.2) in senescing leaves, panicle number, and panicle weight was characterized in rice (Oryza sativa L.). A near-isogenic line (NIL), C-22, developed by marker-assisted selection was grown under different nitrogen levels in the greenhouse and in a paddy field. Chromosome 2 of C-22 had an approximately 50-cM segment substituted from the Kasalath (indica) chromosome in a Koshihikari (japonica) genetic background. C-22 showed a 12-37% lower content of GS1 protein in leaf blades than Koshihikari, which was in good agreement with a QTL region positively affected by the japonica chromosome. At an early vegetative stage, C-22 had more active tillers than Koshihikari in the greenhouse. At the reproductive stage, both panicle number and total panicle weight of C-22 were significantly higher than those of Koshihikari, particularly when the plants were grown under a low-nitrogen condition. These traits of C-22 were further confirmed in a paddy field. Thus, tiller development was positively affected by the Kasalath chromosome at an early vegetative stage, which resulted in an increased panicle number and panicle weight at the mature stage in C-22. These data indicate that the target QTL (Pnn1; panicle number 1) is important in the development of tillers and panicles in rice. Linkage analyses for panicle number and ratio of developing tiller formation in the second axil (RDT) revealed that Pnn1 was delimited at the 6.7-cM region.

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References

Wang X, Woo Y, Kim C, Larkins B . Quantitative trait locus mapping of loci influencing elongation factor 1alpha content in maize endosperm. Plant Physiol. 2001; 125(3):1271-82. PMC: 65607. DOI: 10.1104/pp.125.3.1271. View

Brugiere , Dubois , Limami , Lelandais , Roux , Sangwan . Glutamine synthetase in the phloem plays a major role in controlling proline production. Plant Cell. 1999; 11(10):1995-2012. PMC: 144111. DOI: 10.1105/tpc.11.10.1995. View

Ortega J, Temple S, Sengupta-Gopalan C . Constitutive overexpression of cytosolic glutamine synthetase (GS1) gene in transgenic alfalfa demonstrates that GS1 may be regulated at the level of RNA stability and protein turnover. Plant Physiol. 2001; 126(1):109-21. PMC: 102286. DOI: 10.1104/pp.126.1.109. View

Yano M, Katayose Y, ASHIKARI M, Yamanouchi U, Monna L, Fuse T . Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell. 2001; 12(12):2473-2484. PMC: 102231. DOI: 10.1105/tpc.12.12.2473. View

Loudet O, Chaillou S, Merigout P, Talbotec J, Daniel-Vedele F . Quantitative trait loci analysis of nitrogen use efficiency in Arabidopsis. Plant Physiol. 2003; 131(1):345-58. PMC: 166814. DOI: 10.1104/pp.102.010785. View

Rauh L, Basten C, Buckler 4th S . Quantitative trait loci analysis of growth response to varying nitrogen sources in Arabidopsis thaliana. Theor Appl Genet. 2003; 104(5):743-750. DOI: 10.1007/s00122-001-0815-y. View

Takeda T, Suwa Y, Suzuki M, Kitano H, Ueguchi-Tanaka M, Ashikari M . The OsTB1 gene negatively regulates lateral branching in rice. Plant J. 2003; 33(3):513-20. DOI: 10.1046/j.1365-313x.2003.01648.x. View

Yamaya T, Obara M, Nakajima H, Sasaki S, Hayakawa T, Sato T . Genetic manipulation and quantitative-trait loci mapping for nitrogen recycling in rice. J Exp Bot. 2002; 53(370):917-25. DOI: 10.1093/jexbot/53.370.917. View

Stam M, de Bruin R, van Blokland R, Van der Hoorn R, Mol J, Kooter J . Distinct features of post-transcriptional gene silencing by antisense transgenes in single copy and inverted T-DNA repeat loci. Plant J. 2000; 21(1):27-42. DOI: 10.1046/j.1365-313x.2000.00650.x. View

10.

Hirel B, Bertin P, Quillere I, Bourdoncle W, Attagnant C, Dellay C . Towards a better understanding of the genetic and physiological basis for nitrogen use efficiency in maize. Plant Physiol. 2001; 125(3):1258-70. PMC: 65606. DOI: 10.1104/pp.125.3.1258. View

11.

Li X, Qian Q, Fu Z, Wang Y, Xiong G, Zeng D . Control of tillering in rice. Nature. 2003; 422(6932):618-21. DOI: 10.1038/nature01518. View

12.

Mickelson S, See D, Meyer F, Garner J, Foster C, Blake T . Mapping of QTL associated with nitrogen storage and remobilization in barley (Hordeum vulgare L.) leaves. J Exp Bot. 2003; 54(383):801-12. DOI: 10.1093/jxb/erg084. View

13.

Frary A, Nesbitt T, Grandillo S, Knaap E, Cong B, Liu J . fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science. 2000; 289(5476):85-8. DOI: 10.1126/science.289.5476.85. View

14.

Takahashi Y, Shomura A, Sasaki T, Yano M . Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2. Proc Natl Acad Sci U S A. 2001; 98(14):7922-7. PMC: 35444. DOI: 10.1073/pnas.111136798. View

15.

Dubois F, Brugiere N, Sangwan R, Hirel B . Localization of tobacco cytosolic glutamine synthetase enzymes and the corresponding transcripts shows organ- and cell-specific patterns of protein synthesis and gene expression. Plant Mol Biol. 1996; 31(4):803-17. DOI: 10.1007/BF00019468. View

16.

Ahn S, Tanksley S . Comparative linkage maps of the rice and maize genomes. Proc Natl Acad Sci U S A. 1993; 90(17):7980-4. PMC: 47271. DOI: 10.1073/pnas.90.17.7980. View

17.

Limami A, Rouillon C, Glevarec G, Gallais A, Hirel B . Genetic and physiological analysis of germination efficiency in maize in relation to nitrogen metabolism reveals the importance of cytosolic glutamine synthetase. Plant Physiol. 2002; 130(4):1860-70. PMC: 166697. DOI: 10.1104/pp.009647. View

18.

Vincent R, Fraisier V, Chaillou S, Limami M, Deleens E, Phillipson B . Overexpression of a soybean gene encoding cytosolic glutamine synthetase in shoots of transgenic Lotus corniculatus L. plants triggers changes in ammonium assimilation and plant development. Planta. 1997; 201(4):424-33. DOI: 10.1007/s004250050085. View

19.

Komatsu K, Maekawa M, Ujiie S, Satake Y, Furutani I, Okamoto H . LAX and SPA: major regulators of shoot branching in rice. Proc Natl Acad Sci U S A. 2003; 100(20):11765-70. PMC: 208832. DOI: 10.1073/pnas.1932414100. View

20.

Tanksley S . Mapping polygenes. Annu Rev Genet. 1993; 27:205-33. DOI: 10.1146/annurev.ge.27.120193.001225. View