Dynamic Properties of an Oriented Lipid/DNA Complex Studied by Neutron Scattering

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2004 Nov 16

PMID 15542564

Citations 5

Authors

F Natali

C Castellano

D Pozzi

A Congiu Castellano

Affiliations

Soon will be listed here.

Abstract

The formation of lipid-DNA (CL-DNA) complexes called lipoplexes, proposed as DNA vectors in gene therapy, is obtained by adding DNA to a solution containing liposomes composed of cationic and neutral lipids. The structural and dynamic properties of such lipoplexes are determined by a coupling between the electrostatic interactions and the elastic parameters of the lipid mixture. An attempt to achieve a better understanding of the structure-dynamics relationship is reported herein. In particular, an elastic neutron scattering investigation of DOTAP-DOPC (dioleoyl trimethylammonium propane-dioleoyl phosphatidylcoline) complexed with DNA is described. Proton dynamics in this oriented CL-DNA lipoplex is found to be strongly dependent upon DNA concentration. Our results show that a substantial modification of the membrane dynamics is accompanied by the balancing of the total net charge inside the complex, together with the consequent displacement of interlayer water molecules.

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