Transgenic Mice Expressing a Soluble Form of Porcine Nectin-1/herpesvirus Entry Mediator C As a Model for Pseudorabies-resistant Livestock

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2004 Nov 10

PMID 15534229

Citations 11

Authors

Etsuro Ono

Keiko Amagai

Satoshi Taharaguchi

Yukiko Tomioka

Saori Yoshino

Yuki Watanabe

Pierre Cherel

Louis-Marie Houdebine

Micheline Adam

Marc Eloit

Manabu Inobe

Toshimitsu Uede

Affiliations

Soon will be listed here.

Abstract

An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator C. Nectin-1 is an alpha-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates entry of PRV, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. To assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse lines expressing a soluble form of nectin-1, consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgG1, were generated. All of the transgenic mouse lines showed nearly complete resistance to PRV infection by means of both i.p. and intranasal routes. These results suggest that the introduction into farm animals of a transgene encoding a soluble form of nectin-1 would offer a potent biological approach to generating alpha-herpesvirus-resistant livestock.

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