Detection and Enumeration of Vibrio Vulnificus in Oysters from Two Estuaries Along the Southwest Coast of India, Using Molecular Methods

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2004 Nov 6

PMID 15528563

Citations 17

Authors

Ammini Parvathi

H Sanath Kumar

Indrani Karunasagar

Iddya Karunasagar

Affiliations

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Abstract

This study was conducted to understand the seasonal distribution of Vibrio vulnificus in oysters from two estuaries and the effect of environmental factors on the abundance of V. vulnificus in tropical waters. V. vulnificus was detected in 56.6% of the samples tested by colony hybridization with an alkaline phosphatase-labeled oligonucleotide probe (VV-AP), and the counts ranged from <10/g during the summer months to 10(3)/g in the monsoon season at both sites. The density of V. vulnificus appeared to be controlled more by salinity than by temperature. A nested PCR used in this study detected V. vulnificus in 85% of the samples following 18 h of enrichment in alkaline peptone water.

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