Autologous Grafting with Noncultured Melanocytes: a Simplified Method for Treatment of Depigmented Lesions

Overview

Journal J Am Acad Dermatol

Specialty Dermatology

Date 1992 Feb 1

PMID 1552051

Citations 45

Authors

Y Gauthier

Affiliations

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Abstract

Background: Achromic lesions on the trunk and the extremities often do not respond to treatment and little improvement is obtained in cases of segmental vitiligo.

Objective: Transplantation of autologous noncultured melanocytes was performed to obtain a successful repigmentation.

Methods: The grafting method is carried out in two steps: production of blisters on the depigmented lesions by freezing with liquid nitrogen and injection in each blister of a suspension of epidermal cells (mainly keratinocytes and melanocytes). The cellular suspension was obtained from samples of skin of the hair scalp after trypsinization.

Results: Repigmentation was evident within 25 to 30 days. Coalescence of the pigmented areas was spontaneously observed or obtained after UVA stimulation. Patients with two types of leukoderma-vitiligo or nevus depigmentosus had successful repigmentation after transplantation of autologous noncultured melanocytes.

Conclusion: This technique appears to be an effective and simple method for treating patients with achromic areas lacking melanocytes.

Citing Articles

Efficacy of non-cultured non-trypsinized dermabraded epidermal cell graft followed by narrowband ultraviolet B phototherapy in stable vitiligo.

Chugh D, Kolalapudi S, Praveen S, Konala S, Swetha N, Reddy M J Cutan Aesthet Surg. 2025; 18(1):48-55.

PMID: 40027532 PMC: 11866696. DOI: 10.25259/JCAS_116_2024.

The Efficacy of Non-Cultured Epidermal Cell Suspension and Excimer Lamps Combination Therapy in Vitiligo: Results of 18 Months Follow-Up.

Hoang Van T, Parsad D, Van T, Thi P, Hong S, Thu H J Cosmet Dermatol. 2024; 24(2):e16714.

PMID: 39679850 PMC: 11845954. DOI: 10.1111/jocd.16714.

Sustained Repigmentation in Vitiligo and Leukodermas Using Melanocyte-Keratinocyte Transplantation: 7 Years of Data.

Nuntawisuttiwong N, Yothachai P, Paringkarn T, Chaiyabutr C, Wongpraparut C, Silpa-Archa N Clin Cosmet Investig Dermatol. 2024; 17:2447-2457.

PMID: 39507768 PMC: 11539787. DOI: 10.2147/CCID.S485421.

To study the efficacy of autologous non-cultured nontrypsinized epidermal cell grafting (Jodhpur technique) by using of suction blister membrane in stable vitiligo.

Singh H, Yadav C, Singrodia R, Kachhawa D, Rao P J Cutan Aesthet Surg. 2024; 17(3):239-245.

PMID: 39483660 PMC: 11497548. DOI: 10.25259/jcas_152_22.

A Study of Autologous Non-Cultured Epidermal Suspension (NCES) Transplant in Patients with Stable Vitiligo without the Use of NB-UVB.

Chandela M, Khurana V, Mehta R, Saxena A, Mohanty S, Jethani S Maedica (Bucur). 2024; 18(4):576-585.

PMID: 38348070 PMC: 10859199. DOI: 10.26574/maedica.2023.18.4.576.