Orientation-selected 15N-HYSCORE Detection of Weakly Coupled Nitrogens Around the Archaeal Rieske [2Fe-2S] Center

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2004 Oct 28

PMID 15506733

Citations 12

Authors

Toshio Iwasaki

Asako Kounosu

Taketoshi Uzawa

Rimma I Samoilova

Sergei A Dikanov

Affiliations

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Abstract

The weakly coupled 15N atoms around a reduced Rieske [2Fe-2S] cluster of the uniformly 15N-labeled, hyperthermostable archaeal Rieske protein appear to produce readily observable cross-peaks in the HYSCORE spectra, with the well-resolved couplings of 0.3-0.4 MHz for the Nepsilon and 1.1 MHz for the peptide backbone nitrogens, in addition to the contributions from the coordinated Ndelta atoms. These features can be used for structure-mechanism studies of the biological redox protein system involving the weakly coupled nitrogens in coupled electron-proton transfer reactions.

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