Some Properties of Vacuoles Isolated from Neurospora Crassa Slime Variant

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 1979 Jan 16

PMID 154878

Citations 9

Authors

E Martinoia

U Heck

T Boller

A Wiemken

P Matile

Affiliations

Soon will be listed here.

Abstract

A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described. Isolated vacuoles are characterized by 12 to 50 times increased specific activities of several hydrolases as compared with the total homogenate of protoplasts. Total alpha-amino nitrogen, arginine, and polyphosphate are also greatly enriched in these vacuoles. Vacuoles are equipped with a permease for the transport of basic amino acids across the tonoplast.

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