Stem Cells Expressing Homing Receptors Could Be Expanded from Cryopreserved and Unselected Cord Blood

Overview

Journal J Korean Med Sci

Specialty General Medicine

Date 2004 Oct 16

PMID 15483335

Citations 1

Authors

Young-Ho Lee

Jin-Yeong Han

Su-Yeong Seo

Kyeong-Hee Kim

Young-Ah Lee

Young-Seok Lee

Hyung-Sik Lee

Won-Joo Hur

Hun Han

Hyuk-Chan Kwon

Jae-Seok Kim

Hyo-Jin Kim

Affiliations

Soon will be listed here.

Abstract

We assessed the cytokine combinations that are best for ex vivo expansion of cord blood (CB) and the increment for cell numbers of nucleated cells, as well as stem cells expressing homing receptors, by an ex vivo expansion of cryopreserved and unselected CB. Frozen leukocyte concentrates (LC) from CB were thawed and cultured at a concentration of 1 x 10(5)/mL in media supplemented with a combination of SCF (20 ng/mL)+TPO (50 ng/mL)+FL (50 ng/mL)+/-IL-6 (20 ng/mL)+/-G-CSF (20 ng/mL). After culturing for 14 days, the expansion folds of cell numbers were as follows: TNC 22.3+/-7.8 approximately 26.3+/-4.9, CFU-GM 4.7+/-5.1 approximately 11.7+/-2.6, CD34+CD38- cell 214.0+/-251.9 approximately 464.1+/-566.1, CD34+CXCR4+ cell 4384.5+/-1664.7 approximately 7087.2+/-4669.3, CD34+VLA4+ cell 1444.3+/-1264.0 approximately 2074.9+/-1537.0, CD34+VLA5+ cell 86.2+/-50.9 approximately 113.2+/-57.1. These results revealed that the number of stem cells expressing homing receptors could be increased by an ex vivo expansion of cryopreserved and unselected CB using 3 cytokines (SCF, TPO, FL) only. Further in vivo studies regarding the engraftment after expansion of the nucleated cells, as well as the stem cells expressing homing receptors will be required.

Citing Articles

Cotransplantation of cord blood hematopoietic stem cells and culture-expanded and GM-CSF-/SCF-transfected mesenchymal stem cells in SCID mice.

Han J, Goh R, Seo S, Hwang T, Kwon H, Kim S J Korean Med Sci. 2007; 22(2):242-7.

PMID: 17449931 PMC: 2693589. DOI: 10.3346/jkms.2007.22.2.242.

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