Transepithelial Electrical Measurements with the Ussing Chamber

Overview

Journal J Cyst Fibros

Publisher Elsevier

Specialty Pulmonary Medicine

Date 2004 Oct 7

PMID 15463943

Citations 45

Authors

Hongyu Li

David N Sheppard

Martin J Hug

Affiliations

Soon will be listed here.

Abstract

The Ussing chamber technique is a simple, but powerful technique to investigate ion transport. Originally designed to study vectorial ion transport through the frog skin, it has revolutionized our knowledge about how eletrolytes permeate epithelia. Here we discuss the physiological principles that underlie the technique and protocols to investigate the role of the cystic fibrosis transmembrane conductance regulator (CFTR) in transepithelial ion transport.

Citing Articles

Recent Advances in Studying In Vitro Drug Permeation Across Mucosal Membranes.

Song J, Xu Z, Xie L, Shen J Pharmaceutics. 2025; 17(2).

PMID: 40006623 PMC: 11858820. DOI: 10.3390/pharmaceutics17020256.

In vitro and ex vivo models of the oral mucosa as platforms for the validation of novel drug delivery systems.

Macartney R, Das A, Imaniyyah A, Fricker A, Smith A, Fedele S J Tissue Eng. 2025; 16:20417314241313458.

PMID: 39944725 PMC: 11815840. DOI: 10.1177/20417314241313458.

A Non-Pharmacological Paradigm Captures the Complexity in the Mechanism of Action of Poliprotect Against Gastroesophageal Reflux Disease and Dyspepsia.

Caterbi S, Buttarini C, Garetto S, Franco Moscardini I, Ughetto S, Guerrini A Int J Mol Sci. 2025; 26(3).

PMID: 39940951 PMC: 11818618. DOI: 10.3390/ijms26031181.

Rescue of Mutant CFTR Channel Activity by Investigational Co-Potentiator Therapy.

Bacalhau M, Ferreira F, Azevedo M, Rosa T, Buarque C, Lopes-Pacheco M Biomedicines. 2025; 13(1).

PMID: 39857666 PMC: 11762957. DOI: 10.3390/biomedicines13010082.

Towards Clinical Application: Calcium Waves for In Vitro Qualitative Assessment of Propagated Primary Human Corneal Endothelial Cells.

Ng X, Peh G, Morales-Wong F, Gabriel R, Soong P, Lin K Cells. 2024; 13(23).

PMID: 39682760 PMC: 11640329. DOI: 10.3390/cells13232012.