An Efficient Transformation System for Chickpea (Cicer Arietinum L.)

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2004 Sep 30

PMID 15455257

Citations 15

Authors

G Senthil

B Williamson

R D Dinkins

G Ramsay

Affiliations

Soon will be listed here.

Abstract

A reproducible and efficient transformation method was developed for Desi and Kabuli chickpeas (Cicer arietinum L.) using germinated seedlings as sources of explants. Slices derived from plumules were the most efficient at generating transformed shoots. The AGL1 Agrobacterium-treated explants were first incubated on thidiazuron-containing media, then selected using phosphinothricin. Resistant shoots were successfully transferred to soil either by grafting or in vitro rooting. In experiments each taking 4-9 months, a total of 41 confirmed transformed lines were created using embryo axis slices as source explants, giving a transformation frequency of 5.1%. Southern analysis and histochemical and leaf painting assays demonstrated integration and expression of the transgenes in the initial transformants and two generations of progeny.

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References

Kar S, Johnson T, Nayak P, Sen S . Efficient transgenic plant regeneration throughAgrobacterium-mediated transformation of Chickpea (Cicer arietinum L.). Plant Cell Rep. 2013; 16(1-2):32-7. DOI: 10.1007/BF01275444. View

Schroeder H, Schotz A, Wardley-Richardson T, Spencer D, Higgins T . Transformation and Regeneration of Two Cultivars of Pea (Pisum sativum L.). Plant Physiol. 1993; 101(3):751-757. PMC: 158687. DOI: 10.1104/pp.101.3.751. View

Tewari-Singh N, Sen J, Kiesecker H, Reddy V, Jacobsen H, Guha-Mukherjee S . Use of a herbicide or lysine plus threonine for non-antibiotic selection of transgenic chickpea. Plant Cell Rep. 2004; 22(8):576-83. DOI: 10.1007/s00299-003-0730-6. View

Stam M, de Bruin R, van Blokland R, Van der Hoorn R, Mol J, Kooter J . Distinct features of post-transcriptional gene silencing by antisense transgenes in single copy and inverted T-DNA repeat loci. Plant J. 2000; 21(1):27-42. DOI: 10.1046/j.1365-313x.2000.00650.x. View

Dower W, Miller J, Ragsdale C . High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 1988; 16(13):6127-45. PMC: 336852. DOI: 10.1093/nar/16.13.6127. View

Becker D, Kemper E, Schell J, Masterson R . New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol Biol. 1992; 20(6):1195-7. DOI: 10.1007/BF00028908. View

Ma C, Mitra A . Intrinsic direct repeats generate consistent post-transcriptional gene silencing in tobacco. Plant J. 2002; 31(1):37-49. DOI: 10.1046/j.1365-313x.2002.01332.x. View

Gamborg O, Miller R, Ojima K . Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968; 50(1):151-8. DOI: 10.1016/0014-4827(68)90403-5. View

Vancanneyt G, Schmidt R, Willmitzer L, Rocha-Sosa M . Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet. 1990; 220(2):245-50. DOI: 10.1007/BF00260489. View

10.

McKently A, Moore G, Doostdar H, Niedz R . Agrobacterium-mediated transformation of peanut (Arachis hypogaea L.) embryo axes and the development of transgenic plants. Plant Cell Rep. 2013; 14(11):699-703. DOI: 10.1007/BF00232650. View

11.

Fontana G, Santini L, Caretto S, Frugis G, Mariotti D . Genetic transformation in the grain legume Cicer arietinum L. (chickpea). Plant Cell Rep. 2013; 12(4):194-8. DOI: 10.1007/BF00237052. View

12.

Davies D, Hamilton J, Mullineaux P . Transformation of peas. Plant Cell Rep. 2013; 12(3):180-3. DOI: 10.1007/BF00239102. View

13.

Edwards K, Johnstone C, Thompson C . A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res. 1991; 19(6):1349. PMC: 333874. DOI: 10.1093/nar/19.6.1349. View