Article: Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein

To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2+ SARS-recovered donors at different time points post disease. CD8+DimerX-S1203+ CTLs were detected in the PBMC from these donors up to 3 months after recovery. The percentages of CD8+DimerX-S1203+ cells paralleled the numbers of interferon-gamma-positive spots in an ELISPOT assay using the same antigenic peptide. In conclusion, DimerX-based flow cytometry staining may prove to be a real-time method to screen for CTL directed at epitopes from a newly identified virus.

Citing Articles

SARS-CoV-2 infection and stem cells: Interaction and intervention.

Yu F, Jia R, Tang Y, Liu J, Wei B Stem Cell Res. 2020; 46:101859.

PMID: 32570174 PMC: 7263221. DOI: 10.1016/j.scr.2020.101859.

Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV.

Lv Y, Ruan Z, Wang L, Ni B, Wu Y BMC Immunol. 2009; 10:61.

PMID: 19958537 PMC: 2792222. DOI: 10.1186/1471-2172-10-61.

Long-lived effector/central memory T-cell responses to severe acute respiratory syndrome coronavirus (SARS-CoV) S antigen in recovered SARS patients.

Yang L, Peng H, Zhu Z, Li G, Huang Z, Zhao Z Clin Immunol. 2006; 120(2):171-8.

PMID: 16781892 PMC: 7106132. DOI: 10.1016/j.clim.2006.05.002.

Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine.

Zhao P, Cao J, Zhao L, Qin Z, Ke J, Pan W Virology. 2004; 331(1):128-35.

PMID: 15582659 PMC: 7111813. DOI: 10.1016/j.virol.2004.10.016.

References

1.

Woll M, Fisher C, Ryan G, Gurney J, Storrer C, Ioannides C . Direct measurement of peptide-specific CD8+ T cells using HLA-A2:Ig dimer for monitoring the in vivo immune response to a HER2/neu vaccine in breast and prostate cancer patients. J Clin Immunol. 2004; 24(4):449-61. DOI: 10.1023/B:JOCI.0000029117.10791.98. View

2.

Tanaka Y, Dowdy S, Linehan D, Eberlein T, Goedegebuure P . Induction of antigen-specific CTL by recombinant HIV trans-activating fusion protein-pulsed human monocyte-derived dendritic cells. J Immunol. 2003; 170(3):1291-8. DOI: 10.4049/jimmunol.170.3.1291. View

3.

Wang Y, Xie Y, Chen W . [Immunoinformatic analysis for the epitopes on SARS virus surface protein]. Beijing Da Xue Xue Bao Yi Xue Ban. 2003; 35 Suppl:70-1. View

4.

Lew T, Kwek T, Tai D, Earnest A, Loo S, Singh K . Acute respiratory distress syndrome in critically ill patients with severe acute respiratory syndrome. JAMA. 2003; 290(3):374-80. DOI: 10.1001/jama.290.3.374. View

5.

Gricks C, Rawlings E, Foroni L, Madrigal J, Amlot P . Somatically mutated regions of immunoglobulin on human B-cell lymphomas code for peptides that bind to autologous major histocompatibility complex class I, providing a potential target for cytotoxic T cells. Cancer Res. 2001; 61(13):5145-52. View

Detecting Specific Cytotoxic T Lymphocytes Against SARS-coronavirus with DimerX HLA-A2:Ig Fusion Protein