Expression of Viral EPS-depolymerase Reduces Fire Blight Susceptibility in Transgenic Pear

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2004 Sep 18

PMID 15375629

Citations 10

Authors

Mickael Malnoy

Mohamed Faize

Jean-Stephane Venisse

Klaus Geider

Elisabeth Chevreau

Affiliations

Soon will be listed here.

Abstract

Erwinia amylovora is the causal agent of fire blight of Maloideae. One of the main pathogenicity factors of this bacterium is the exopolysaccharide (EPS) of its capsule. In this paper, we used genetic transformation tools to constitutively express an EPS-depolymerase transgene in the pear (Pyrus communis L.) cv. Passe Crassane with the aim of decreasing its high susceptibility to fire blight. Expression of the depolymerase gene in 15 independent transgenic clones led, on average, to low depolymerase activity, although relatively high expression was observed at the transcriptional and translational levels. Only two of the transgenic clones (9X and 10M) consistently showed a decrease in fire blight susceptibility in vitro and in the greenhouse. These clones were also among the highest expressers of depolymerase at the RNA and enzyme activity levels. The correlation observed among all transgenic clones between depolymerase expression and fire blight resistance suggested the potential of this strategy.

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