NG2-positive Cells in the Mouse White and Grey Matter Display Distinct Physiological Properties

Overview

Journal J Physiol

Specialty Physiology

Date 2004 Sep 11

PMID 15358811

Citations 121

Authors

R Chittajallu

A Aguirre

V Gallo

Affiliations

Soon will be listed here.

Abstract

Cells that express the NG2 proteoglycan are the largest proliferative progenitor population in the postnatal central nervous system (CNS). Although this entire population has long been considered to be oligodendrocyte progenitors, numerous NG2(+) cells are present in the cerebral cortex, where relatively little myelination occurs, and also persist long after myelination is complete in the CNS. Several studies have alluded to the presence of distinct NG2(+) cell subtypes based on marker expression, but no experimentally derived hypotheses about the physiological role of these subtypes has been proposed. In the current study, whole-cell patch-clamp data from acutely isolated slices demonstrate that subcortical white matter and cortical NG2(+) cells display distinct membrane properties in addition to possessing differing K(+)- and Na(+)-channel expression profiles. A striking observation is that a subpopulation of cortical, but not white matter NG2(+) cells, elicit depolarization-induced spikes that are akin to immature action potentials. Our data demonstrate that a population of cortical NG2(+) cells display physiological properties that differ from their white matter counterparts.

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References

Kotecha S, Schlichter L . A Kv1.5 to Kv1.3 switch in endogenous hippocampal microglia and a role in proliferation. J Neurosci. 1999; 19(24):10680-93. PMC: 6784954. View

Berry M, Hubbard P, Butt A . Cytology and lineage of NG2-positive glia. J Neurocytol. 2003; 31(6-7):457-67. DOI: 10.1023/a:1025735513560. View

Magavi S, Leavitt B, Macklis J . Induction of neurogenesis in the neocortex of adult mice. Nature. 2000; 405(6789):951-5. DOI: 10.1038/35016083. View

MacFarlane S, Sontheimer H . Modulation of Kv1.5 currents by Src tyrosine phosphorylation: potential role in the differentiation of astrocytes. J Neurosci. 2000; 20(14):5245-53. PMC: 6772320. View

Dawson M, Levine J, Reynolds R . NG2-expressing cells in the central nervous system: are they oligodendroglial progenitors?. J Neurosci Res. 2000; 61(5):471-9. DOI: 10.1002/1097-4547(20000901)61:5<471::AID-JNR1>3.0.CO;2-N. View

Kondo T, Raff M . Oligodendrocyte precursor cells reprogrammed to become multipotential CNS stem cells. Science. 2000; 289(5485):1754-7. DOI: 10.1126/science.289.5485.1754. View

Levine J, Reynolds R, Fawcett J . The oligodendrocyte precursor cell in health and disease. Trends Neurosci. 2001; 24(1):39-47. DOI: 10.1016/s0166-2236(00)01691-x. View

Kirchhoff F, Kettenmann H, Levine J, Trotter J . AN2/NG2 protein-expressing glial progenitor cells in the murine CNS: isolation, differentiation, and association with radial glia. Glia. 2001; 34(3):213-28. DOI: 10.1002/glia.1055. View

Neusch C, Rozengurt N, Jacobs R, Lester H, Kofuji P . Kir4.1 potassium channel subunit is crucial for oligodendrocyte development and in vivo myelination. J Neurosci. 2001; 21(15):5429-38. PMC: 6762664. View

10.

He W, Ingraham C, Rising L, Goderie S, Temple S . Multipotent stem cells from the mouse basal forebrain contribute GABAergic neurons and oligodendrocytes to the cerebral cortex during embryogenesis. J Neurosci. 2001; 21(22):8854-62. PMC: 6762260. View

11.

Gage F . Neurogenesis in the adult brain. J Neurosci. 2002; 22(3):612-3. PMC: 6758482. View

12.

Rakic P . Adult neurogenesis in mammals: an identity crisis. J Neurosci. 2002; 22(3):614-8. PMC: 6758501. View

13.

Mallon B, Shick H, Kidd G, Macklin W . Proteolipid promoter activity distinguishes two populations of NG2-positive cells throughout neonatal cortical development. J Neurosci. 2002; 22(3):876-85. PMC: 6758537. View

14.

Chittajallu R, Chen Y, Wang H, Yuan X, Ghiani C, Heckman T . Regulation of Kv1 subunit expression in oligodendrocyte progenitor cells and their role in G1/S phase progression of the cell cycle. Proc Natl Acad Sci U S A. 2002; 99(4):2350-5. PMC: 122368. DOI: 10.1073/pnas.042698399. View

15.

Braun H, Schafer K, Hollt V . BetaIII tubulin-expressing neurons reveal enhanced neurogenesis in hippocampal and cortical structures after a contusion trauma in rats. J Neurotrauma. 2002; 19(8):975-83. DOI: 10.1089/089771502320317122. View

16.

Gipson K, Bordey A . Analysis of the K+ current profile of mature rat oligodendrocytes in situ. J Membr Biol. 2002; 189(3):201-12. DOI: 10.1007/s00232-002-1014-8. View

17.

Yuan X, Chittajallu R, Belachew S, Anderson S, McBain C, Gallo V . Expression of the green fluorescent protein in the oligodendrocyte lineage: a transgenic mouse for developmental and physiological studies. J Neurosci Res. 2002; 70(4):529-45. DOI: 10.1002/jnr.10368. View

18.

Matthias K, Kirchhoff F, Seifert G, Huttmann K, Matyash M, Kettenmann H . Segregated expression of AMPA-type glutamate receptors and glutamate transporters defines distinct astrocyte populations in the mouse hippocampus. J Neurosci. 2003; 23(5):1750-8. PMC: 6741945. View

19.

Nunes M, Roy N, Keyoung H, Goodman R, McKhann 2nd G, Jiang L . Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain. Nat Med. 2003; 9(4):439-47. DOI: 10.1038/nm837. View

20.

Picken Bahrey H, Moody W . Early development of voltage-gated ion currents and firing properties in neurons of the mouse cerebral cortex. J Neurophysiol. 2003; 89(4):1761-73. DOI: 10.1152/jn.00972.2002. View