Somatic Cell Mapping of the Bovine Interferon-alpha Receptor

Overview

Journal Mamm Genome

Specialty Genetics

Date 1992 Jan 1

PMID 1535250

Citations 2

Authors

J A Langer

R Puvanakrishnan

J E Womack

Affiliations

Soon will be listed here.

Abstract

The bovine interferon-alpha receptor (BoIFN-alpha R) mediates the activity of bovine IFN-alpha s and IFN-beta. In addition, human IFN-alpha s have uniformly high biological activity on bovine cells. A 32P-labeled derivative of human recombinant IFN-alpha A (HuIFN-alpha A-P1) binds well and can form a characteristic 130-kDa complex on bovine cells, but not on hamster cells. We have, therefore, analyzed the binding and covalent crosslinking of [32P]HuIFN-alpha A-P1 to a panel of bovine-hamster somatic cell hybrids. Binding to several bovine-hamster hybrid cell lines was strong (about 30-50% of that seen with bovine MDBK cells) and specific. The binding correlated uniquely with bovine syntenic group U10. In several of the hybrid lines, the ability of human IFN-alpha B to enhance the expression of endogenous MHC class I molecules correlated with the binding results. We thus conclude that the bovine IFN-alpha R structural gene (locus designation IFNAR) localizes to syntenic group U10. This group includes a number of other genes whose homologs map to human Chromosome (Chr) 21.

Citing Articles

Impact of the Type I Interferon Receptor on the Global Gene Expression Program During the Course of Dendritic Cell Maturation Induced by Polyinosinic Polycytidylic Acid.

Olex A, Turkett W, Brzoza-Lewis K, Fetrow J, Hiltbold E J Interferon Cytokine Res. 2016; 36(6):382-400.

PMID: 27035059 PMC: 4892211. DOI: 10.1089/jir.2014.0150.

The bovine genome map.

Fries R, Eggen A, Womack J Mamm Genome. 1993; 4(8):405-28.

PMID: 8104056 DOI: 10.1007/BF00296815.

References

Shulman L, Kamarck M, Slate D, Ruddle F, BRANCA A, Baglioni C . Antibodies to chromosome 21 coded cell surface components block binding of human alpha interferon but not gamma interferon to human cells. Virology. 1984; 137(2):422-7. DOI: 10.1016/0042-6822(84)90235-6. View

Trent J, Olson S, Lawn R . Chromosomal localization of human leukocyte, fibroblast, and immune interferon genes by means of in situ hybridization. Proc Natl Acad Sci U S A. 1982; 79(24):7809-13. PMC: 347438. DOI: 10.1073/pnas.79.24.7809. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Henco K, Brosius J, Fujisawa A, Fujisawa J, Haynes J, Hochstadt J . Structural relationship of human interferon alpha genes and pseudogenes. J Mol Biol. 1985; 185(2):227-60. DOI: 10.1016/0022-2836(85)90401-2. View

Raziuddin A, Sarkar F, Dutkowski R, Shulman L, Ruddle F, Gupta S . Receptors for human alpha and beta interferon but not for gamma interferon are specified by human chromosome 21. Proc Natl Acad Sci U S A. 1984; 81(17):5504-8. PMC: 391734. DOI: 10.1073/pnas.81.17.5504. View

Ullrich A, Gray A, Goeddel D, Dull T . Nucleotide sequence of a portion of human chromosome 9 containing a leukocyte interferon gene cluster. J Mol Biol. 1982; 156(3):467-86. DOI: 10.1016/0022-2836(82)90261-3. View

Staehelin T, Hobbs D, Kung H, Lai C, Pestka S . Purification and characterization of recombinant human leukocyte interferon (IFLrA) with monoclonal antibodies. J Biol Chem. 1981; 256(18):9750-4. View

Kelley K, Kozak C, Dandoy F, Sor F, Skup D, Windass J . Mapping of murine interferon-alpha genes to chromosome 4. Gene. 1983; 26(2-3):181-8. DOI: 10.1016/0378-1119(83)90188-9. View

McAvin J, Patterson D, Womack J . Mapping of bovine PRGS and PAIS genes in hybrid somatic cells: syntenic conservation with human chromosome 21. Biochem Genet. 1988; 26(1-2):9-18. DOI: 10.1007/BF00555485. View

10.

Womack J, Moll Y . Gene map of the cow: conservation of linkage with mouse and man. J Hered. 1986; 77(1):2-7. DOI: 10.1093/oxfordjournals.jhered.a110160. View

11.

Lovett M, Cox D, Yee D, Boll W, Weissmann C, Epstein C . The chromosomal location of mouse interferon alpha genes. EMBO J. 1984; 3(7):1643-6. PMC: 557571. DOI: 10.1002/j.1460-2075.1984.tb02023.x. View

12.

Revel M, Bash D, Ruddle F . Antibodies to a cell-surface component coded by human chromosome 21 inhibit action of interferon. Nature. 1976; 260(5547):139-41. DOI: 10.1038/260139a0. View

13.

Lin P, Slate D, Lawyer F, Ruddle F . Assignment of the murine interferon sensitivity and cytoplasmic superoxide dismutase genes to chromosome 16. Science. 1980; 209(4453):285-7. DOI: 10.1126/science.6155698. View

14.

Cox D, Epstein L, Epstein C . Genes coding for sensitivity to interferon (IfRec) and soluble superoxide dismutase (SOD-1) are linked in mouse and man and map to mouse chromosome 16. Proc Natl Acad Sci U S A. 1980; 77(4):2168-72. PMC: 348673. DOI: 10.1073/pnas.77.4.2168. View

15.

Langer J, Pestka S . Interferon receptors. Immunol Today. 1988; 9(12):393-400. DOI: 10.1016/0167-5699(88)91241-8. View

16.

Aguet M, Dembic Z, Merlin G . Molecular cloning and expression of the human interferon-gamma receptor. Cell. 1988; 55(2):273-80. DOI: 10.1016/0092-8674(88)90050-5. View

17.

Rashidbaigi A, Langer J, Jung V, Jones C, Morse H, Tischfield J . The gene for the human immune interferon receptor is located on chromosome 6. Proc Natl Acad Sci U S A. 1986; 83(2):384-8. PMC: 322863. DOI: 10.1073/pnas.83.2.384. View

18.

Tan Y, Tischfield J, Ruddle F . The linkage of genes for the human interferon-induced antiviral protein and indophenol oxidase-B traits to chromosome G-21. J Exp Med. 1973; 137(2):317-30. PMC: 2139494. DOI: 10.1084/jem.137.2.317. View

19.

Cahilly L, George D, Daugherty B, Pestka S . Subchromosomal localization of mouse IFN-alpha genes by in situ hybridization. J Interferon Res. 1985; 5(3):391-5. DOI: 10.1089/jir.1985.5.391. View

20.

van der Korput J, Hilkens J, Kroezen V, Zwarthoff E, Trapman J . Mouse interferon alpha and beta genes are linked at the centromere proximal region of chromosome 4. J Gen Virol. 1985; 66 ( Pt 3):493-502. DOI: 10.1099/0022-1317-66-3-493. View