Reduced Protein Adsorption at Solid Interfaces by Sugar Excipients

Overview

Journal Biotechnol Bioeng

Publisher Wiley

Specialty Biochemistry

Date 2004 Sep 8

PMID 15352054

Citations 10

Authors

Janet R Wendorf

Clayton J Radke

Harvey W Blanch

Affiliations

Soon will be listed here.

Abstract

Sugar excipients are shown to reduce the adsorption of ribonuclease A, bovine serum albumin, and hen egg white lysozyme at the liquid-solid interface. The amount of protein adsorbed decreased as the concentration of the sugar increased. At the same sugar concentration, the ability of sugars to reduce protein adsorption followed the trend: trisaccharides > disaccharides > 6-carbon polyols > monosaccharides. This trend in adsorbed protein amounts among sugars was explained by stabilization of the protein native state in solution by the sugar excipients. The heat of solution of the amorphous saccharide was found to correlate with the amount of protein adsorbed.

Citing Articles

Pulmonary Inhalation of Biotherapeutics: A Systematic Approach to Understanding the Effects of Atomisation Gas Flow Rate on Particle Physiochemical Properties and Retained Bioactivity.

Foley L, Ziaee A, Walker G, OReilly E Pharmaceutics. 2024; 16(8).

PMID: 39204365 PMC: 11359500. DOI: 10.3390/pharmaceutics16081020.

Pharmaceutical protein solids: Drying technology, solid-state characterization and stability.

Chen Y, Tejasvi Mutukuri T, Wilson N, Zhou Q Adv Drug Deliv Rev. 2021; 172:211-233.

PMID: 33705880 PMC: 8107147. DOI: 10.1016/j.addr.2021.02.016.

Osmolytes: A Possible Therapeutic Molecule for Ameliorating the Neurodegeneration Caused by Protein Misfolding and Aggregation.

Kushwah N, Jain V, Yadav D Biomolecules. 2020; 10(1).

PMID: 31941036 PMC: 7022651. DOI: 10.3390/biom10010132.

Understanding the Role of Preferential Exclusion of Sugars and Polyols from Native State IgG1 Monoclonal Antibodies and its Effect on Aggregation and Reversible Self-Association.

Sudrik C, Cloutier T, Mody N, Sathish H, Trout B Pharm Res. 2019; 36(8):109.

PMID: 31127417 DOI: 10.1007/s11095-019-2642-3.

Inhibition of insulin fibrillation by osmolytes: Mechanistic insights.

Choudhary S, Kishore N, Hosur R Sci Rep. 2015; 5:17599.

PMID: 26616401 PMC: 4663473. DOI: 10.1038/srep17599.