In Vitro Growth and Differentiation of Osteoblast-like Human Bone Marrow Cells on Glass Reinforced Hydroxyapatite Plasma-sprayed Coatings

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2004 Sep 7

PMID 15348109

Citations 3

Authors

M P Ferraz

M H Fernandes

A Trigo Cabral

J D Santos

F J Monteiro

Affiliations

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Abstract

Human osteoblastic bone marrow cells were cultured for periods of up to 28 days in control conditions and on the surface of a glass reinforced hydroxyapatite composite (HA/G1) and commercial hydroxyapatite (HA) plasma-sprayed coatings, in the "as-received" condition and after immersion treatment in culture medium for 21 days. Cultures were characterized for total protein content and alkaline phosphatase activity. Scanning electron microscope analyses were performed on control cultures, seeded materials and materials incubated in the absence of cells. Culture media were analyzed for total and ionized calcium and phosphorus concentrations throughout the incubation period. Immersion of HA/G1 and HA coatings in culture medium resulted in significant alterations to the levels of calcium and phosphorus in the medium, leading to surface modifications. However, seeded material samples showed significant differences in the pattern of variation of the levels of these species. Cell proliferation was observed in the "as-received" HA/G1 composite, but cell mediated formation of mineral deposits was not proved. In contrast, "as-received" HA hardly supported cell growth. Previously immersed material samples showed cell proliferation and evidence of biological formation of mineral deposits. However, the HA/G1 composite presented better surface characteristics for cell growth as the behavior of bone marrow cells was closer to that observed in control cultures.

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References

Tomas H, Carvalho G, Fernandes M, Freire A, Abrantes L . The use of rat, rabbit or human bone marrow derived cells for cytocompatibility evaluation of metallic elements. J Mater Sci Mater Med. 1997; 8(4):233-8. DOI: 10.1023/a:1018543808210. View

Nagase M, Abe Y, Chigira M, UDAGAWA E . Toxicity of silica-containing calcium phosphate glasses demonstrated in mice. Biomaterials. 1992; 13(3):172-5. DOI: 10.1016/0142-9612(92)90067-x. View

Courteney-Harris R, Kayser M, Downes S . Comparison of the early production of extracellular matrix on dense hydroxyapatite and hydroxyapatite-coated titanium in cell and organ culture. Biomaterials. 1995; 16(6):489-95. DOI: 10.1016/0142-9612(95)98823-w. View

Morais S, Dias N, Sousa J, Fernandes M, Carvalho G . In vitro osteoblastic differentiation of human bone marrow cells in the presence of metal ions. J Biomed Mater Res. 1999; 44(2):176-90. DOI: 10.1002/(sici)1097-4636(199902)44:2<176::aid-jbm8>3.0.co;2-6. View

Ong J, Prince C, Lucas L . Cellular response to well-characterized calcium phosphate coatings and titanium surfaces in vitro. J Biomed Mater Res. 1995; 29(2):165-72. DOI: 10.1002/jbm.820290205. View

Daculsi G, LeGeros R, Heughebaert M, Barbieux I . Formation of carbonate-apatite crystals after implantation of calcium phosphate ceramics. Calcif Tissue Int. 1990; 46(1):20-7. DOI: 10.1007/BF02555820. View

Fernandes M, Costa M, Carvalho G . Mineralization in serially passaged human alveolar bone cells. J Mater Sci Mater Med. 1997; 8(2):61-5. DOI: 10.1023/a:1018598430983. View

de Bruijn J, Klein C, de Groot K, van Blitterswijk C . The ultrastructure of the bone-hydroxyapatite interface in vitro. J Biomed Mater Res. 1992; 26(10):1365-82. DOI: 10.1002/jbm.820261008. View

Owen T, Aronow M, Shalhoub V, Barone L, Wilming L, Tassinari M . Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol. 1990; 143(3):420-30. DOI: 10.1002/jcp.1041430304. View

10.

Maniatopoulos C, Sodek J, Melcher A . Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res. 1988; 254(2):317-30. DOI: 10.1007/BF00225804. View

11.

Aronow M, Gerstenfeld L, Owen T, Tassinari M, Stein G, Lian J . Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells. J Cell Physiol. 1990; 143(2):213-21. DOI: 10.1002/jcp.1041430203. View

12.

Santos J, Jha L, Monteiro F . Surface modifications of glass-reinforced hydroxyapatite composites. Biomaterials. 1995; 16(7):521-6. DOI: 10.1016/0142-9612(95)91124-h. View

13.

Best S, Sim B, Kayser M, Downes S . The dependence of osteoblastic response on variations in the chemical composition and physical properties of hydroxyapatite. J Mater Sci Mater Med. 1997; 8(2):97-103. DOI: 10.1023/a:1018558816871. View

14.

Labat B, Chamson A, Frey J . Effects of gamma-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts. J Biomed Mater Res. 1995; 29(11):1397-401. DOI: 10.1002/jbm.820291111. View

15.

Anselme K, Sharrock P, Hardouin P, Dard M . In vitro growth of human adult bone-derived cells on hydroxyapatite plasma-sprayed coatings. J Biomed Mater Res. 1997; 34(2):247-59. DOI: 10.1002/(sici)1097-4636(199702)34:2<247::aid-jbm14>3.0.co;2-f. View

16.

Amedee J, Bareille R, Jeandot R, Bordenave L, Remy M, Rouais F . Evaluation of cell colonization on biomaterials: preventing cell attachment to plastic containers. Biomaterials. 1994; 15(12):1029-31. DOI: 10.1016/0142-9612(94)90086-8. View

17.

de Bruijn J, van Blitterswijk C, Davies J . Initial bone matrix formation at the hydroxyapatite interface in vivo. J Biomed Mater Res. 1995; 29(1):89-99. DOI: 10.1002/jbm.820290113. View

18.

Gundle R, Joyner C, Triffitt J . Human bone tissue formation in diffusion chamber culture in vivo by bone-derived cells and marrow stromal fibroblastic cells. Bone. 1995; 16(6):597-601. DOI: 10.1016/8756-3282(95)00112-q. View

19.

Kirkpatrick C, Wagner M, Kohler H, Bittinger F, Otto M, Klein C . The cell and molecular biological approach to biomaterial research: a perspective. J Mater Sci Mater Med. 1997; 8(3):131-41. DOI: 10.1023/a:1018567018688. View

20.

Bagambisa F, Joos U, SCHILLI W . Mechanisms and structure of the bond between bone and hydroxyapatite ceramics. J Biomed Mater Res. 1993; 27(8):1047-55. DOI: 10.1002/jbm.820270810. View