Incoherent Manipulation of the Photoactive Yellow Protein Photocycle with Dispersed Pump-dump-probe Spectroscopy

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2004 Sep 4

PMID 15345564

Citations 29

Authors

Delmar S Larsen

Ivo H M van Stokkum

Mikas Vengris

Michael A van der Horst

Frank L de Weerd

Klaas J Hellingwerf

Rienk van Grondelle

Affiliations

Soon will be listed here.

Abstract

Photoactive yellow protein is the protein responsible for initiating the "blue-light vision" of Halorhodospira halophila. The dynamical processes responsible for triggering the photoactive yellow protein photocycle have been disentangled with the use of a novel application of dispersed ultrafast pump-dump-probe spectroscopy, where the photocycle can be started and interrupted with appropriately tuned and timed laser pulses. This "incoherent" manipulation of the photocycle allows for the detailed spectroscopic investigation of the underlying photocycle dynamics and the construction of a fully self-consistent dynamical model. This model requires three kinetically distinct excited-state intermediates, two (ground-state) photocycle intermediates, I(0) and pR, and a ground-state intermediate through which the protein, after unsuccessful attempts at initiating the photocycle, returns to the equilibrium ground state. Also observed is a previously unknown two-photon ionization channel that generates a radical and an ejected electron into the protein environment. This second excitation pathway evolves simultaneously with the pathway containing the one-photon photocycle intermediates.

Citing Articles

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