Comparative Mapping Between Medicago Sativa and Pisum Sativum

Overview

Journal Mol Genet Genomics

Specialty Genetics

Date 2004 Sep 2

PMID 15340836

Citations 62

Authors

P Kalo

A Seres

S A Taylor

J Jakab

Z Kevei

A Kereszt

G Endre

T H N Ellis

G B Kiss

Affiliations

Soon will be listed here.

Abstract

Comparative genome analysis has been performed between alfalfa (Medicago sativa) and pea (Pisum sativum), species which represent two closely related tribes of the subfamily Papilionoideae with different basic chromosome numbers. The positions of genes on the most recent linkage map of diploid alfalfa were compared to those of homologous loci on the combined genetic map of pea to analyze the degree of co-linearity between their linkage groups. In addition to using unique genes, analysis of the map positions of multicopy (homologous) genes identified syntenic homologs (characterized by similar positions on the maps) and pinpointed the positions of non-syntenic homologs. The comparison revealed extensive conservation of gene order between alfalfa and pea. However, genetic rearrangements (due to breakage and reunion) were localized which can account for the difference in chromosome number (8 for alfalfa and 7 for pea). Based on these genetic events and our increasing knowledge of the genomic structure of pea, it was concluded that the difference in genome size between the two species (the pea genome is 5- to 10-fold larger than that of alfalfa) is not a consequence of genome duplication in pea. The high degree of synteny observed between pea and Medicago loci makes further map-based cloning of pea genes based on the genome resources now available for M. truncatula a promising strategy.

Citing Articles

Underutilized legumes: nutrient status and advanced breeding approaches for qualitative and quantitative enhancement.

Samal I, Bhoi T, Raj M, Majhi P, Murmu S, Pradhan A Front Nutr. 2023; 10:1110750.

PMID: 37275642 PMC: 10232757. DOI: 10.3389/fnut.2023.1110750.

Mendel: From genes to genome.

Sussmilch F, Ross J, Reid J Plant Physiol. 2022; 190(4):2103-2114.

PMID: 36094356 PMC: 9706470. DOI: 10.1093/plphys/kiac424.

The genetic architecture of flowering time changes in pea from wild to crop.

Williams O, Vander Schoor J, Butler J, Ridge S, Sussmilch F, Hecht V J Exp Bot. 2022; 73(12):3978-3990.

PMID: 35383838 PMC: 9238443. DOI: 10.1093/jxb/erac132.

The Cowpea Kinome: Genomic and Transcriptomic Analysis Under Biotic and Abiotic Stresses.

Ferreira-Neto J, Borges A, Silva M, Morais D, Bezerra-Neto J, Bourque G Front Plant Sci. 2021; 12:667013.

PMID: 34194450 PMC: 8238008. DOI: 10.3389/fpls.2021.667013.

Genetic and Molecular Genetic Basis of Nuclear-Plastid Incompatibilities.

Bogdanova V Plants (Basel). 2019; 9(1).

PMID: 31878042 PMC: 7020172. DOI: 10.3390/plants9010023.

References

Ehrlich J, Sankoff D, Nadeau J . Synteny conservation and chromosome rearrangements during mammalian evolution. Genetics. 1997; 147(1):289-96. PMC: 1208112. DOI: 10.1093/genetics/147.1.289. View

Hofer J, Turner L, Hellens R, Ambrose M, Matthews P, Michael A . UNIFOLIATA regulates leaf and flower morphogenesis in pea. Curr Biol. 1997; 7(8):581-7. DOI: 10.1016/s0960-9822(06)00257-0. View

Gowri G, Paiva N, Dixon R . Stress responses in alfalfa (Medicago sativa L.) 12. Sequence analysis of phenylalanine ammonia-lyase (PAL) cDNA clones and appearance of PAL transcripts in elicitor-treated cell cultures and developing plants. Plant Mol Biol. 1991; 17(3):415-29. DOI: 10.1007/BF00040636. View

Harker C, Ellis T, Coen E . Identification and genetic regulation of the chalcone synthase multigene family in pea. Plant Cell. 1990; 2(3):185-94. PMC: 159875. DOI: 10.1105/tpc.2.3.185. View

Ellis T, Turner L, Hellens R, Lee D, Harker C, Enard C . Linkage maps in pea. Genetics. 1992; 130(3):649-63. PMC: 1204881. DOI: 10.1093/genetics/130.3.649. View

Tanksley S, Ganal M, Prince J, de Vicente M, Bonierbale M, Broun P . High density molecular linkage maps of the tomato and potato genomes. Genetics. 1992; 132(4):1141-60. PMC: 1205235. DOI: 10.1093/genetics/132.4.1141. View

Brummer E, Bouton J, Kochert G . Development of an RFLP map in diploid alfalfa. Theor Appl Genet. 2013; 86(2-3):329-32. DOI: 10.1007/BF00222097. View

Hall K, Parker J, Ellis T, Turner L, Knox M, Hofer J . The relationship between genetic and cytogenetic maps of pea. II. Physical maps of linkage mapping populations. Genome. 2008; 40(5):755-69. DOI: 10.1139/g97-798. View

Tanksley S, Bernatzky R, Lapitan N, Prince J . Conservation of gene repertoire but not gene order in pepper and tomato. Proc Natl Acad Sci U S A. 1988; 85(17):6419-23. PMC: 281983. DOI: 10.1073/pnas.85.17.6419. View

10.

Turner S, Ireland R, Rawsthorne S . Purification and primary amino acid sequence of the L subunit of glycine decarboxylase. Evidence for a single lipoamide dehydrogenase in plant mitochondria. J Biol Chem. 1992; 267(11):7745-50. View

11.

Kiss G, Csanadi G, Kalman K, Kalo P, Okresz L . Construction of a basic genetic map for alfalfa using RFLP, RAPD, isozyme and morphological markers. Mol Gen Genet. 1993; 238(1-2):129-37. DOI: 10.1007/BF00279539. View

12.

Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T . Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci U S A. 1989; 86(8):2766-70. PMC: 286999. DOI: 10.1073/pnas.86.8.2766. View

13.

Boivin K, Acarkan A, Mbulu R, Clarenz O, Schmidt R . The Arabidopsis genome sequence as a tool for genome analysis in Brassicaceae. A comparison of the Arabidopsis and Capsella rubella genomes. Plant Physiol. 2004; 135(2):735-44. PMC: 514111. DOI: 10.1104/pp.104.040030. View

14.

Acarkan A, ROSSBERG M, Koch M, Schmidt R . Comparative genome analysis reveals extensive conservation of genome organisation for Arabidopsis thaliana and Capsella rubella. Plant J. 2000; 23(1):55-62. DOI: 10.1046/j.1365-313x.2000.00790.x. View

15.

Vincent J, Knox M, Ellis T, Kalo P, Kiss G, Brewin N . Nodule-expressed Cyp15a cysteine protease genes map to syntenic genome regions in Pisum and Medicago spp. Mol Plant Microbe Interact. 2000; 13(7):715-23. DOI: 10.1094/MPMI.2000.13.7.715. View

16.

Bennetzen J, SanMiguel P, Chen M, Tikhonov A, Francki M, Avramova Z . Grass genomes. Proc Natl Acad Sci U S A. 1998; 95(5):1975-8. PMC: 33825. DOI: 10.1073/pnas.95.5.1975. View

17.

Choi H, Mun J, Kim D, Zhu H, Baek J, Mudge J . Estimating genome conservation between crop and model legume species. Proc Natl Acad Sci U S A. 2004; 101(43):15289-94. PMC: 524433. DOI: 10.1073/pnas.0402251101. View

18.

Macherel D, Bourguignon J, Douce R . Cloning of the gene (gdcH) encoding H-protein, a component of the glycine decarboxylase complex of pea (Pisum sativum L.). Biochem J. 1992; 286 ( Pt 2):627-30. PMC: 1132945. DOI: 10.1042/bj2860627. View

19.

Kulikova O, Gualtieri G, Geurts R, Kim D, Cook D, Huguet T . Integration of the FISH pachytene and genetic maps of Medicago truncatula. Plant J. 2001; 27(1):49-58. DOI: 10.1046/j.1365-313x.2001.01057.x. View

20.

Devos K, Gale M . Comparative genetics in the grasses. Plant Mol Biol. 1997; 35(1-2):3-15. View