Y Enriched and Y Specific DNA Sequences from the Genome of the Mediterranean Fruit Fly, Ceratitis Capitata

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1992 Mar 1

PMID 1533581

Citations 8

Authors

J E Anleitner

D S Haymer

Affiliations

Soon will be listed here.

Abstract

DNA sequences that are enriched or specific to the genome of the male medfly, Ceratitis capitata, have been isolated using a differential hybridization approach. Twelve phage clones from a genomic library have been identified that consistently display more intense hybridization with a genomic DNA probe from males as opposed to one from females. Southern DNA blot analysis reveals that these recombinant clones contain at least one EcoRI fragment that is either specific to the male genome, or more highly represented in it, as compared with the female genome. These EcoRI fragments, when used as probes, all generate a similar pattern of intense multiple bands in genomic DNA of males. This suggests the presence of repetitive sequences that are at least partially homologous in these regions of the genome that are specific to or enriched in males. In situ hybridization to mitotic chromosomes confirms a Y chromosomal origin for the male specific repetitive sequences. Data on the genomic organization, representation and evolutionary conservation of these sequences that are specific to or enriched in males are presented. Studies of the genomic organization and representation of flanking sequences that are not male specific are presented as well.

Citing Articles

Female-to-male sex conversion in Ceratitis capitata by CRISPR/Cas9 HDR-induced point mutations in the sex determination gene transformer-2.

Aumann R, Hacker I, Schetelig M Sci Rep. 2020; 10(1):18611.

PMID: 33122768 PMC: 7596080. DOI: 10.1038/s41598-020-75572-x.

The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species.

Papanicolaou A, Schetelig M, Arensburger P, Atkinson P, Benoit J, Bourtzis K Genome Biol. 2016; 17(1):192.

PMID: 27659211 PMC: 5034548. DOI: 10.1186/s13059-016-1049-2.

Achilles, a New Family of Transcriptionally Active Retrotransposons from the Olive Fruit Fly, with Y Chromosome Preferential Distribution.

Tsoumani K, Drosopoulou E, Bourtzis K, Gariou-Papalexiou A, Mavragani-Tsipidou P, Zacharopoulou A PLoS One. 2015; 10(9):e0137050.

PMID: 26398504 PMC: 4580426. DOI: 10.1371/journal.pone.0137050.

Subtractive and differential hybridization molecular analyses of Ceratitis capitata XX/XY versus XX embryos to search for male-specific early transcribed genes.

Salvemini M, DAmato R, Petrella V, Ippolito D, Ventre G, Zhang Y BMC Genet. 2014; 15 Suppl 2:S5.

PMID: 25472628 PMC: 4255797. DOI: 10.1186/1471-2156-15-S2-S5.

Interchromosomal duplications on the Bactrocera oleae Y chromosome imply a distinct evolutionary origin of the sex chromosomes compared to Drosophila.

Gabrieli P, Gomulski L, Bonomi A, Siciliano P, Scolari F, Franz G PLoS One. 2011; 6(3):e17747.

PMID: 21408187 PMC: 3049792. DOI: 10.1371/journal.pone.0017747.

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