Cellular Immune Responses to the Murine Nematode Parasite Trichuris Muris. II. Differential Induction of TH-cell Subsets in Resistant Versus Susceptible Mice

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1992 Feb 1

PMID 1532377

Citations 56

Authors

K J Else

L Hultner

R K Grencis

Affiliations

Soon will be listed here.

Abstract

We have analysed the production of a wide variety of cytokines by in vitro concanavalin A (Con A) stimulated mesenteric lymph node cells (MLNC) from strains of mice experiencing chronic (B10.BR, AKR) versus acute (BALB/K) infection with the nematode parasite Trichuris muris. MLNC from infected BALB/K mice produced elevated levels of the Th2-specific cytokines interleukin-5 (IL-5) and IL-9. IL-3 and IL-4 remained at or just above normal. Interferon-gamma (IFN-gamma) (a Th1-type cytokine) was secreted only in small amounts. MLNC from infected susceptible B10.BR and AKR mice produced large amounts of IFN-gamma in the relative absence of IL-4 and IL-9. IL-5 levels failed to rise significantly above normal in B10.BR mice whilst in AKR mice high levels of IL-5 were detected early post-infection (p.i.) but levels decreased dramatically as the infection proceeded to reach normal levels by Day 34. IL-3 levels were depressed below normal. Our results are consistent with the polarization of the Th-cell response during T. muris infection to give predominantly IFN-gamma-secreting Th1 cells in strains of mice unable to expel the parasite and mainly IL-4, IL-5 and IL-9 producing Th2-type cells in resistant strains.

Citing Articles

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References

Uyttenhove C, Simpson R, Van Snick J . Functional and structural characterization of P40, a mouse glycoprotein with T-cell growth factor activity. Proc Natl Acad Sci U S A. 1988; 85(18):6934-8. PMC: 282093. DOI: 10.1073/pnas.85.18.6934. View

Smith K . Interleukin-2: inception, impact, and implications. Science. 1988; 240(4856):1169-76. DOI: 10.1126/science.3131876. View

Lee T, Wakelin D, Grencis R . Cellular mechanisms of immunity to the nematode Trichuris muris. Int J Parasitol. 1983; 13(4):349-53. DOI: 10.1016/s0020-7519(83)80039-3. View

Pearce E, Caspar P, Grzych J, Lewis F, Sher A . Downregulation of Th1 cytokine production accompanies induction of Th2 responses by a parasitic helminth, Schistosoma mansoni. J Exp Med. 1991; 173(1):159-66. PMC: 2118762. DOI: 10.1084/jem.173.1.159. View

Else K, Grencis R . Cellular immune responses to the murine nematode parasite Trichuris muris. I. Differential cytokine production during acute or chronic infection. Immunology. 1991; 72(4):508-13. PMC: 1384369. View

Ito Y . The absence of resistance in congenitally athymic nude mice toward infection with the intestinal nematode, Trichuris muris: resistance restored by lymphoid cell transfer. Int J Parasitol. 1991; 21(1):65-9. DOI: 10.1016/0020-7519(91)90121-m. View

Van Snick J . Interleukin-6: an overview. Annu Rev Immunol. 1990; 8:253-78. DOI: 10.1146/annurev.iy.08.040190.001345. View

Heinzel F, Sadick M, Holaday B, Coffman R, Locksley R . Reciprocal expression of interferon gamma or interleukin 4 during the resolution or progression of murine leishmaniasis. Evidence for expansion of distinct helper T cell subsets. J Exp Med. 1989; 169(1):59-72. PMC: 2189187. DOI: 10.1084/jem.169.1.59. View

Fiorentino D, Bond M, Mosmann T . Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones. J Exp Med. 1989; 170(6):2081-95. PMC: 2189521. DOI: 10.1084/jem.170.6.2081. View

10.

Van Snick J, Cayphas S, Vink A, Uyttenhove C, Coulie P, Rubira M . Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas. Proc Natl Acad Sci U S A. 1986; 83(24):9679-83. PMC: 387204. DOI: 10.1073/pnas.83.24.9679. View

11.

Cherwinski H, Schumacher J, Brown K, Mosmann T . Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med. 1987; 166(5):1229-44. PMC: 2189643. DOI: 10.1084/jem.166.5.1229. View

12.

Gajewski T, Fitch F . Anti-proliferative effect of IFN-gamma in immune regulation. I. IFN-gamma inhibits the proliferation of Th2 but not Th1 murine helper T lymphocyte clones. J Immunol. 1988; 140(12):4245-52. View

13.

Else K, Wakelin D . The effects of H-2 and non-H-2 genes on the expulsion of the nematode Trichuris muris from inbred and congenic mice. Parasitology. 1988; 96 ( Pt 3):543-50. DOI: 10.1017/s0031182000080173. View

14.

Murray P, McKenzie D, Swain S, Kagnoff M . Interleukin 5 and interleukin 4 produced by Peyer's patch T cells selectively enhance immunoglobulin A expression. J Immunol. 1987; 139(8):2669-74. View

15.

Wakelin D . Acquired immunity to Trichuris muris in the albino laboratory mouse. Parasitology. 1967; 57(3):515-24. DOI: 10.1017/s0031182000072395. View

16.

Greenberger J, Sakakeeny M, Humphries R, Eaves C, Eckner R . Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines. Proc Natl Acad Sci U S A. 1983; 80(10):2931-5. PMC: 393947. DOI: 10.1073/pnas.80.10.2931. View

17.

Lee T, Wakelin D . The use of host strain variation to assess the significance of mucosal mast cells in the spontaneous cure response of mice to the nematode Trichuris muris. Int Arch Allergy Appl Immunol. 1982; 67(4):302-5. DOI: 10.1159/000233037. View

18.

Pond L, Wassom D, Hayes C . Evidence for differential induction of helper T cell subsets during Trichinella spiralis infection. J Immunol. 1989; 143(12):4232-7. View

19.

Smith C, Rennick D . Characterization of a murine lymphokine distinct from interleukin 2 and interleukin 3 (IL-3) possessing a T-cell growth factor activity and a mast-cell growth factor activity that synergizes with IL-3. Proc Natl Acad Sci U S A. 1986; 83(6):1857-61. PMC: 323183. DOI: 10.1073/pnas.83.6.1857. View

20.

Gillis S, Ferm M, Ou W, Smith K . T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978; 120(6):2027-32. View