Auxin-induced SCFTIR1-Aux/IAA Interaction Involves Stable Modification of the SCFTIR1 Complex

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2004 Aug 6

PMID 15295098

Citations 68

Authors

Stefan Kepinski

Ottoline Leyser

Affiliations

Soon will be listed here.

Abstract

The plant hormone auxin can regulate gene expression by destabilizing members of the Aux/IAA family of transcriptional repressors. Auxin-induced Aux/IAA degradation requires the protein-ubiquitin ligase SCF(TIR1), with auxin acting to enhance the interaction between the Aux/IAAs and SCF(TIR1). SKP1, Cullin, and an F-box-containing protein (SCF)-mediated degradation is an important component of many eukaryotic signaling pathways. In all known cases to date, the interaction between the targets and their cognate SCFs is regulated by signal-induced modification of the target. The mechanism by which auxin promotes the interaction between SCF(TIR1) and Aux/IAAs is not understood, but current hypotheses propose auxin-induced phosphorylation, hydroxylation, or proline isomerization of the Aux/IAAs. We found no evidence to support these hypotheses or indeed that auxin induces any stable modification of Aux/IAAs to increase their affinity for SCF(TIR1). Instead, we present data suggesting that auxin promotes the SCF(TIR1)-Aux/IAA interaction by affecting the SCF component, TIR1, or proteins tightly associated with it.

Citing Articles

The Double-Stranded RNA Binding Proteins DRB1 and DRB2 Are Required for miR160-Mediated Responses to Exogenous Auxin.

Zimmerman K, Pegler J, Oultram J, Collings D, Wang M, Grof C Genes (Basel). 2025; 15(12.

PMID: 39766914 PMC: 11675975. DOI: 10.3390/genes15121648.

Exploring Genomic Regions Associated with Fruit Traits in Pepper: Insights from Multiple GWAS Models.

Ro N, Oh H, Ko H, Yi J, Na Y, Haile M Int J Mol Sci. 2024; 25(21).

PMID: 39519386 PMC: 11546569. DOI: 10.3390/ijms252111836.

Genetically Encoded, Noise-Tolerant, Auxin Biosensors in Yeast.

Chaisupa P, Rahman M, Hildreth S, Moseley S, Gatling C, Bryant M ACS Synth Biol. 2024; 13(9):2804-2819.

PMID: 39197086 PMC: 11421217. DOI: 10.1021/acssynbio.4c00186.

Slow and rapid auxin responses in Arabidopsis.

Zhang Z, Chen H, Peng S, Han H J Exp Bot. 2024; 75(18):5471-5476.

PMID: 38794966 PMC: 11427834. DOI: 10.1093/jxb/erae246.

regulates adventitious root development in via an auxin-mediated pathway.

Wei P, Lv Y, Guang Q, Han J, Wang Y, Wang X Plant Signal Behav. 2023; 18(1):2218670.

PMID: 37288791 PMC: 10251782. DOI: 10.1080/15592324.2023.2218670.

References

Deshaies R . SCF and Cullin/Ring H2-based ubiquitin ligases. Annu Rev Cell Dev Biol. 1999; 15:435-67. DOI: 10.1146/annurev.cellbio.15.1.435. View

Gray W, Del Pozo J, Walker L, Hobbie L, Risseeuw E, Banks T . Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana. Genes Dev. 1999; 13(13):1678-91. PMC: 316846. DOI: 10.1101/gad.13.13.1678. View

Ouellet F, Overvoorde P, Theologis A . IAA17/AXR3: biochemical insight into an auxin mutant phenotype. Plant Cell. 2001; 13(4):829-41. PMC: 135541. DOI: 10.1105/tpc.13.4.829. View

Ivan M, Kondo K, Yang H, Kim W, Valiando J, Ohh M . HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing. Science. 2001; 292(5516):464-8. DOI: 10.1126/science.1059817. View

Jaakkola P, Mole D, Tian Y, Wilson M, Gielbert J, Gaskell S . Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science. 2001; 292(5516):468-72. DOI: 10.1126/science.1059796. View

Zenser N, Ellsmore A, Leasure C, Callis J . Auxin modulates the degradation rate of Aux/IAA proteins. Proc Natl Acad Sci U S A. 2001; 98(20):11795-800. PMC: 58810. DOI: 10.1073/pnas.211312798. View

Ramos J, Zenser N, Leyser O, Callis J . Rapid degradation of auxin/indoleacetic acid proteins requires conserved amino acids of domain II and is proteasome dependent. Plant Cell. 2001; 13(10):2349-60. PMC: 139163. DOI: 10.1105/tpc.010244. View

Gray W, Kepinski S, Rouse D, Leyser O, Estelle M . Auxin regulates SCF(TIR1)-dependent degradation of AUX/IAA proteins. Nature. 2001; 414(6861):271-6. DOI: 10.1038/35104500. View

Tiwari S, Wang X, Hagen G, Guilfoyle T . AUX/IAA proteins are active repressors, and their stability and activity are modulated by auxin. Plant Cell. 2001; 13(12):2809-22. PMC: 139490. DOI: 10.1105/tpc.010289. View

10.

Kepinski S, Leyser O . Ubiquitination and auxin signaling: a degrading story. Plant Cell. 2002; 14 Suppl:S81-95. PMC: 151249. View

11.

Yoshida Y, Chiba T, Tokunaga F, Kawasaki H, Iwai K, Suzuki T . E3 ubiquitin ligase that recognizes sugar chains. Nature. 2002; 418(6896):438-42. DOI: 10.1038/nature00890. View

12.

Dharmasiri N, Dharmasiri S, Jones A, Estelle M . Auxin action in a cell-free system. Curr Biol. 2003; 13(16):1418-22. DOI: 10.1016/s0960-9822(03)00536-0. View

13.

Zhao Y, Dai X, Blackwell H, Schreiber S, Chory J . SIR1, an upstream component in auxin signaling identified by chemical genetics. Science. 2003; 301(5636):1107-10. DOI: 10.1126/science.1084161. View

14.

Tian Q, Nagpal P, Reed J . Regulation of Arabidopsis SHY2/IAA3 protein turnover. Plant J. 2003; 36(5):643-51. DOI: 10.1046/j.1365-313x.2003.01909.x. View

15.

Abel S, Theologis A . Early genes and auxin action. Plant Physiol. 1996; 111(1):9-17. PMC: 157808. DOI: 10.1104/pp.111.1.9. View

16.

Ruegger M, Dewey E, Gray W, Hobbie L, Turner J, Estelle M . The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast grr1p. Genes Dev. 1998; 12(2):198-207. PMC: 316440. DOI: 10.1101/gad.12.2.198. View

17.

Rouse D, MacKay P, Stirnberg P, Estelle M, Leyser O . Changes in auxin response from mutations in an AUX/IAA gene. Science. 1998; 279(5355):1371-3. DOI: 10.1126/science.279.5355.1371. View

18.

Hennig L, Christner C, Kipping M, Schelbert B, Rucknagel K, Grabley S . Selective inactivation of parvulin-like peptidyl-prolyl cis/trans isomerases by juglone. Biochemistry. 1998; 37(17):5953-60. DOI: 10.1021/bi973162p. View

19.

Ulmasov T, Hagen G, Guilfoyle T . Activation and repression of transcription by auxin-response factors. Proc Natl Acad Sci U S A. 1999; 96(10):5844-9. PMC: 21948. DOI: 10.1073/pnas.96.10.5844. View

20.

Berleth T, Sachs T . Plant morphogenesis: long-distance coordination and local patterning. Curr Opin Plant Biol. 2001; 4(1):57-62. DOI: 10.1016/s1369-5266(00)00136-9. View