Cloning and Characterization of a Sixth Adenylyl Cyclase Isoform: Types V and VI Constitute a Subgroup Within the Mammalian Adenylyl Cyclase Family

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1992 Sep 15

PMID 1528892

Citations 54

Authors

S Katsushika

L Chen

J Kawabe

R Nilakantan

N J Halnon

C J Homcy

Y Ishikawa

Affiliations

Soon will be listed here.

Abstract

A sixth member of the mammalian adenylyl cyclase family has been isolated from a canine cardiac cDNA library. This isoform is more highly homologous to type V than to the other adenylyl cyclase types; sequence similarity is apparent even in the transmembrane regions where the greatest divergence among the types exists. Type VI mRNA expression is most abundant in heart and brain; however, unlike type V, a low level of expression is also observed in a variety of other tissues examined. Type VI adenylyl cyclase can be stimulated by NaF, guanosine 5'-[gamma-thio]triphosphate, and forskolin but not by Ca2+/calmodulin, whereas it is inhibited by adenosine and its analogues. Comparison of both their structural and biochemical properties suggests that types V and VI constitute a distinct subgroup of the mammalian adenylyl cyclase family.

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References

Krupinski J, Coussen F, Bakalyar H, Tang W, Feinstein P, Orth K . Adenylyl cyclase amino acid sequence: possible channel- or transporter-like structure. Science. 1989; 244(4912):1558-64. DOI: 10.1126/science.2472670. View

Kozak M . The scanning model for translation: an update. J Cell Biol. 1989; 108(2):229-41. PMC: 2115416. DOI: 10.1083/jcb.108.2.229. View

Kataoka T, Broek D, Wigler M . DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase. Cell. 1985; 43(2 Pt 1):493-505. DOI: 10.1016/0092-8674(85)90179-5. View

Gerard R, Gluzman Y . New host cell system for regulated simian virus 40 DNA replication. Mol Cell Biol. 1985; 5(11):3231-40. PMC: 369139. DOI: 10.1128/mcb.5.11.3231-3240.1985. View

Feng D, Doolittle R . Progressive sequence alignment as a prerequisite to correct phylogenetic trees. J Mol Evol. 1987; 25(4):351-60. DOI: 10.1007/BF02603120. View

Pustell J . Interactive molecular biology computing. Nucleic Acids Res. 1988; 16(5):1813-20. PMC: 338175. DOI: 10.1093/nar/16.5.1813. View

Gros P, Croop J, Housman D . Mammalian multidrug resistance gene: complete cDNA sequence indicates strong homology to bacterial transport proteins. Cell. 1986; 47(3):371-80. DOI: 10.1016/0092-8674(86)90594-5. View

Bourne H, Beiderman B, Steinberg F, Brothers V . Three adenylate cyclase phenotypes in S49 lymphoma cells produced by mutations of one gene. Mol Pharmacol. 1982; 22(1):204-10. View

Devereux J, Haeberli P, SMITHIES O . A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984; 12(1 Pt 1):387-95. PMC: 321012. DOI: 10.1093/nar/12.1part1.387. View

10.

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

11.

Ishikawa Y, Katsushika S, Chen L, Halnon N, Kawabe J, Homcy C . Isolation and characterization of a novel cardiac adenylylcyclase cDNA. J Biol Chem. 1992; 267(19):13553-7. View

12.

Tang W, Krupinski J, GILMAN A . Expression and characterization of calmodulin-activated (type I) adenylylcyclase. J Biol Chem. 1991; 266(13):8595-603. View

13.

Feldman M, Copelas L, Gwathmey J, Phillips P, Warren S, Schoen F . Deficient production of cyclic AMP: pharmacologic evidence of an important cause of contractile dysfunction in patients with end-stage heart failure. Circulation. 1987; 75(2):331-9. DOI: 10.1161/01.cir.75.2.331. View

14.

Chinkers M, Garbers D, Chang M, Lowe D, Chin H, Goeddel D . A membrane form of guanylate cyclase is an atrial natriuretic peptide receptor. Nature. 1989; 338(6210):78-83. DOI: 10.1038/338078a0. View

15.

Rommens J, Iannuzzi M, Kerem B, Drumm M, Melmer G, Dean M . Identification of the cystic fibrosis gene: chromosome walking and jumping. Science. 1989; 245(4922):1059-65. DOI: 10.1126/science.2772657. View

16.

Homcy C, Vatner S, Vatner D . Beta-adrenergic receptor regulation in the heart in pathophysiologic states: abnormal adrenergic responsiveness in cardiac disease. Annu Rev Physiol. 1991; 53:137-59. DOI: 10.1146/annurev.ph.53.030191.001033. View

17.

Feinstein P, Schrader K, Bakalyar H, Tang W, Krupinski J, GILMAN A . Molecular cloning and characterization of a Ca2+/calmodulin-insensitive adenylyl cyclase from rat brain. Proc Natl Acad Sci U S A. 1991; 88(22):10173-7. PMC: 52890. DOI: 10.1073/pnas.88.22.10173. View

18.

Gao B, GILMAN A . Cloning and expression of a widely distributed (type IV) adenylyl cyclase. Proc Natl Acad Sci U S A. 1991; 88(22):10178-82. PMC: 52891. DOI: 10.1073/pnas.88.22.10178. View

19.

Ishikawa Y, Bianchi C, Nadal-Ginard B, Homcy C . Alternative promoter and 5' exon generate a novel Gs alpha mRNA. J Biol Chem. 1990; 265(15):8458-62. View

20.

Bakalyar H, Reed R . Identification of a specialized adenylyl cyclase that may mediate odorant detection. Science. 1990; 250(4986):1403-6. DOI: 10.1126/science.2255909. View