Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2004 Jul 16

PMID 15254166

Citations 30

Authors

Rannveig Larsen

Torunn P Rokenes

Borre Robertsen

Affiliations

Soon will be listed here.

Abstract

Mx proteins form a family of interferon (IFN)-induced GTPases with potent antiviral activity against various single-stranded RNA viruses in mammals and chickens. In fish, alpha/beta IFN has been reported to inhibit the replication of infectious pancreatic necrosis virus (IPNV), but the mode of action has not been elucidated. A correlation between the inhibition of IPNV and Mx protein expression has, however, been observed. To examine whether Atlantic salmon Mx1 protein (ASMx1) possesses antiviral activity against IPNV, CHSE-214 cells constitutively expressing ASMx1 were established. ASMx1 appeared to be localized in the cytoplasm. The ASMx1-expressing clone selected showed a severely reduced IPNV-induced cytopathic effect, which was confirmed by a 500-fold reduction in virus yield. The antiviral activity against IPNV was further confirmed by the inhibition of virus protein synthesis and the reduced accumulation of virus transcripts. The present work further adds to the body of evidence which suggests that antiviral activity is a major functional role of vertebrate Mx proteins. Moreover, the list of viruses inhibited by Mx proteins is extended to include double-stranded RNA viruses.

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References

Lee J, Hirono I, Aoki T . Cloning and analysis of expression of Mx cDNA in Japanese flounder, Paralichthys olivaceus. Dev Comp Immunol. 2000; 24(4):407-15. DOI: 10.1016/s0145-305x(99)00060-9. View

Jensen V, Robertsen B . Cloning of an Mx cDNA from Atlantic halibut (Hippoglossus hippoglossus) and characterization of Mx mRNA expression in response to double-stranded RNA or infectious pancreatic necrosis virus. J Interferon Cytokine Res. 2000; 20(8):701-10. DOI: 10.1089/10799900050116408. View

Nygaard R, Husgard S, Sommer A, Leong J, Robertsen B . Induction of Mx protein by interferon and double-stranded RNA in salmonid cells. Fish Shellfish Immunol. 2000; 10(5):435-50. DOI: 10.1006/fsim.1999.0249. View

Goodbourn S, Didcock L, Randall R . Interferons: cell signalling, immune modulation, antiviral response and virus countermeasures. J Gen Virol. 2000; 81(Pt 10):2341-2364. DOI: 10.1099/0022-1317-81-10-2341. View

Gordien E, Rosmorduc O, Peltekian C, Garreau F, Brechot C, Kremsdorf D . Inhibition of hepatitis B virus replication by the interferon-inducible MxA protein. J Virol. 2001; 75(6):2684-91. PMC: 115893. DOI: 10.1128/JVI.75.6.2684-2691.2001. View

Ellis A . Innate host defense mechanisms of fish against viruses and bacteria. Dev Comp Immunol. 2001; 25(8-9):827-39. DOI: 10.1016/s0145-305x(01)00038-6. View

Kochs G, Janzen C, Hohenberg H, Haller O . Antivirally active MxA protein sequesters La Crosse virus nucleocapsid protein into perinuclear complexes. Proc Natl Acad Sci U S A. 2002; 99(5):3153-8. PMC: 122488. DOI: 10.1073/pnas.052430399. View

Lee S, Vidal S . Functional diversity of Mx proteins: variations on a theme of host resistance to infection. Genome Res. 2002; 12(4):527-30. DOI: 10.1101/gr.20102. View

Ko J, Jin H, Asano A, Takada A, Ninomiya A, Kida H . Polymorphisms and the differential antiviral activity of the chicken Mx gene. Genome Res. 2002; 12(4):595-601. PMC: 187515. DOI: 10.1101/gr.210702. View

10.

Haller O, Kochs G . Interferon-induced mx proteins: dynamin-like GTPases with antiviral activity. Traffic. 2002; 3(10):710-7. DOI: 10.1034/j.1600-0854.2002.31003.x. View

11.

Jensen I, Robertsen B . Effect of double-stranded RNA and interferon on the antiviral activity of Atlantic salmon cells against infectious salmon anemia virus and infectious pancreatic necrosis virus. Fish Shellfish Immunol. 2002; 13(3):221-41. DOI: 10.1006/fsim.2001.0397. View

12.

Jensen I, Larsen R, Robertsen B . An antiviral state induced in Chinook salmon embryo cells (CHSE-214) by transfection with the double-stranded RNA poly I:C. Fish Shellfish Immunol. 2002; 13(5):367-78. DOI: 10.1006/fsim.2002.0412. View

13.

Asano A, Ko J, Morozumi T, Hamashima N, Watanabe T . Polymorphisms and the antiviral property of porcine Mx1 protein. J Vet Med Sci. 2003; 64(12):1085-9. DOI: 10.1292/jvms.64.1085. View

14.

Altmann S, Mellon M, Distel D, Kim C . Molecular and functional analysis of an interferon gene from the zebrafish, Danio rerio. J Virol. 2003; 77(3):1992-2002. PMC: 140984. DOI: 10.1128/jvi.77.3.1992-2002.2003. View

15.

Yap W, Tay A, Brenner S, Venkatesh B . Molecular cloning of the pufferfish (Takifugu rubripes) Mx gene and functional characterization of its promoter. Immunogenetics. 2003; 54(10):705-13. DOI: 10.1007/s00251-002-0525-x. View

16.

Long S, Wilson M, Bengten E, Bryan L, Clem L, Miller N . Identification of a cDNA encoding channel catfish interferon. Dev Comp Immunol. 2003; 28(2):97-111. DOI: 10.1016/s0145-305x(03)00122-8. View

17.

Robertsen B, Bergan V, Rokenes T, Larsen R, Albuquerque A . Atlantic salmon interferon genes: cloning, sequence analysis, expression, and biological activity. J Interferon Cytokine Res. 2003; 23(10):601-12. DOI: 10.1089/107999003322485107. View

18.

Caipang C, Hirono I, Aoki T . In vitro inhibition of fish rhabdoviruses by Japanese flounder, Paralichthys olivaceus Mx. Virology. 2003; 317(2):373-82. DOI: 10.1016/j.virol.2003.08.040. View

19.

Plant K, Thune R . Cloning and characterisation of a channel catfish (Ictalurus punctatus) Mx gene. Fish Shellfish Immunol. 2004; 16(3):391-405. DOI: 10.1016/j.fsi.2003.07.001. View

20.

Horisberger M, Staeheli P, Haller O . Interferon induces a unique protein in mouse cells bearing a gene for resistance to influenza virus. Proc Natl Acad Sci U S A. 1983; 80(7):1910-4. PMC: 393720. DOI: 10.1073/pnas.80.7.1910. View