Lack of Homologous Sequence-specific DNA Methylation in Response to Stable DsRNA Expression in Mouse Oocytes

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2004 Jul 13

PMID 15247344

Citations 13

Authors

Petr Svoboda

Paula Stein

Witold Filipowicz

Richard M Schultz

Affiliations

Soon will be listed here.

Abstract

Double-stranded RNA (dsRNA) induces sequence-specific mRNA degradation in most eukaryotic organisms via a conserved pathway known as RNA interference (RNAi). Post-transcriptional gene silencing by RNAi is also connected with transcriptional silencing of cognate sequences. In plants, this transcriptional silencing is associated with sequence-specific DNA methylation. To address whether this mechanism operates in mammalian cells, we used bisulfite sequencing to analyze DNA in mouse oocytes constitutively expressing long dsRNA against the Mos gene. Our data show that long dsRNA induces efficient Mos mRNA knockdown but not CpG and non-CpG DNA methylation of the endogenous Mos sequence in oocytes and early embryos. These data demonstrate that dsRNA does not directly induce DNA methylation in the trans form of this sequence in these mammalian cells.

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References

Ramsahoye B, Biniszkiewicz D, Lyko F, Clark V, Bird A, Jaenisch R . Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a. Proc Natl Acad Sci U S A. 2000; 97(10):5237-42. PMC: 25812. DOI: 10.1073/pnas.97.10.5237. View

Lucifero D, Mertineit C, Clarke H, Bestor T, Trasler J . Methylation dynamics of imprinted genes in mouse germ cells. Genomics. 2002; 79(4):530-8. DOI: 10.1006/geno.2002.6732. View

Mette M, Aufsatz W, van der Winden J, Matzke M, Matzke A . Transcriptional silencing and promoter methylation triggered by double-stranded RNA. EMBO J. 2000; 19(19):5194-201. PMC: 302106. DOI: 10.1093/emboj/19.19.5194. View

Morel J, Mourrain P, Beclin C, Vaucheret H . DNA methylation and chromatin structure affect transcriptional and post-transcriptional transgene silencing in Arabidopsis. Curr Biol. 2001; 10(24):1591-4. DOI: 10.1016/s0960-9822(00)00862-9. View

Bartee L, Malagnac F, Bender J . Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene. Genes Dev. 2001; 15(14):1753-8. PMC: 312734. DOI: 10.1101/gad.905701. View

Svoboda P, Stein P, Schultz R . RNAi in mouse oocytes and preimplantation embryos: effectiveness of hairpin dsRNA. Biochem Biophys Res Commun. 2001; 287(5):1099-104. DOI: 10.1006/bbrc.2001.5707. View

Bourchis D, Xu G, Lin C, Bollman B, Bestor T . Dnmt3L and the establishment of maternal genomic imprints. Science. 2001; 294(5551):2536-9. DOI: 10.1126/science.1065848. View

Obata Y, Kono T . Maternal primary imprinting is established at a specific time for each gene throughout oocyte growth. J Biol Chem. 2001; 277(7):5285-9. DOI: 10.1074/jbc.M108586200. View

Cao X, Jacobsen S . Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing. Curr Biol. 2002; 12(13):1138-44. DOI: 10.1016/s0960-9822(02)00925-9. View

10.

Volpe T, Kidner C, Hall I, Teng G, Grewal S, Martienssen R . Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi. Science. 2002; 297(5588):1833-7. DOI: 10.1126/science.1074973. View

11.

Hall I, Shankaranarayana G, Noma K, Ayoub N, Cohen A, Grewal S . Establishment and maintenance of a heterochromatin domain. Science. 2002; 297(5590):2232-7. DOI: 10.1126/science.1076466. View

12.

Aufsatz W, Mette M, van der Winden J, Matzke A, Matzke M . RNA-directed DNA methylation in Arabidopsis. Proc Natl Acad Sci U S A. 2002; 99 Suppl 4:16499-506. PMC: 139914. DOI: 10.1073/pnas.162371499. View

13.

Lane N, Dean W, Erhardt S, Hajkova P, Surani A, Walter J . Resistance of IAPs to methylation reprogramming may provide a mechanism for epigenetic inheritance in the mouse. Genesis. 2003; 35(2):88-93. DOI: 10.1002/gene.10168. View

14.

Zilberman D, Cao X, Jacobsen S . ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation. Science. 2003; 299(5607):716-9. DOI: 10.1126/science.1079695. View

15.

Stein P, Svoboda P, Schultz R . Transgenic RNAi in mouse oocytes: a simple and fast approach to study gene function. Dev Biol. 2003; 256(1):187-93. DOI: 10.1016/s0012-1606(02)00122-7. View

16.

Tufarelli C, Stanley J, Garrick D, Sharpe J, Ayyub H, Wood W . Transcription of antisense RNA leading to gene silencing and methylation as a novel cause of human genetic disease. Nat Genet. 2003; 34(2):157-65. DOI: 10.1038/ng1157. View

17.

Fedoriw A, Stein P, Svoboda P, Schultz R, Bartolomei M . Transgenic RNAi reveals essential function for CTCF in H19 gene imprinting. Science. 2004; 303(5655):238-40. DOI: 10.1126/science.1090934. View

18.

Yu J, Deng M, Medvedev S, Yang J, Hecht N, Schultz R . Transgenic RNAi-mediated reduction of MSY2 in mouse oocytes results in reduced fertility. Dev Biol. 2004; 268(1):195-206. DOI: 10.1016/j.ydbio.2003.12.020. View

19.

Lucifero D, Mann M, Bartolomei M, Trasler J . Gene-specific timing and epigenetic memory in oocyte imprinting. Hum Mol Genet. 2004; 13(8):839-49. DOI: 10.1093/hmg/ddh104. View

20.

La Salle S, Mertineit C, Taketo T, Moens P, Bestor T, Trasler J . Windows for sex-specific methylation marked by DNA methyltransferase expression profiles in mouse germ cells. Dev Biol. 2004; 268(2):403-15. DOI: 10.1016/j.ydbio.2003.12.031. View