Rapid Discrimination Between Strains of Beta Haemolytic Streptococci by Intact Cell Mass Spectrometry

Overview

Journal Indian J Med Res

Specialty General Medicine

Date 2004 Jul 10

PMID 15243166

Citations 7

Authors

M Palani Kumar

M Vairamani

Raju Prasada Raju

Charmaine Lobo

N Anbumani

C P Girish Kumar

Thangam Menon

S Shanmugasundaram

Affiliations

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Abstract

Background & Objectives: Beta haemolytic streptococci belonging to Lancefield group A, B, C and G cause a wide spectrum of clinical diseases. Hence there is a need for rapid and accurate typing of these strains. The present study was undertaken to evaluate the use of intact cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for rapid discrimination between strains of beta haemolytic streptococci.

Methods: Colonies of beta haemolytic streptococci were emulsified with chemical matrix on the sample slide, dried and analyzed by MALDI-TOF-MS.

Results: The reproducibility of results for all groups of beta haemolytic streptococci was good and spectra obtained for Lancefield group A, C and G streptococci showed discrimination between the groups on visual comparison. A finer difference in spectrum was observed among group A streptococci isolated from different locations at different periods of time.

Interpretation & Conclusion: MALDI-TOF-MS may be a potential tool in discriminating between strains of beta haemolytic streptococci, and also in the characterisation of untypable strains of group A streptococci.

Citing Articles

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.

Singhal N, Kumar M, Kanaujia P, Virdi J Front Microbiol. 2015; 6:791.

PMID: 26300860 PMC: 4525378. DOI: 10.3389/fmicb.2015.00791.

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of beta-hemolytic streptococci.

Zhou C, Tao L, Hu B, Ma J, Ye X, Huang S J Thorac Dis. 2015; 7(4):591-5.

PMID: 25973224 PMC: 4419311. DOI: 10.3978/j.issn.2072-1439.2015.03.02.

Discrimination of multilocus sequence typing-based Campylobacter jejuni subgroups by MALDI-TOF mass spectrometry.

Zautner A, Masanta W, Tareen A, Weig M, Lugert R, Gross U BMC Microbiol. 2013; 13:247.

PMID: 24195572 PMC: 4228279. DOI: 10.1186/1471-2180-13-247.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology.

Clark A, Kaleta E, Arora A, Wolk D Clin Microbiol Rev. 2013; 26(3):547-603.

PMID: 23824373 PMC: 3719498. DOI: 10.1128/CMR.00072-12.

Evaluation of species-specific score cutoff values of routinely isolated clinically relevant bacteria using a direct smear preparation for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based bacterial identification.

Szabados F, Tix H, Anders A, Kaase M, Gatermann S, Geis G Eur J Clin Microbiol Infect Dis. 2011; 31(6):1109-19.

PMID: 21948378 DOI: 10.1007/s10096-011-1415-7.