Validation of a PCR-based Method for Detection of Food-borne Thermotolerant Campylobacters in a Multicenter Collaborative Trial

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2004 Jul 9

PMID 15240324

Citations 5

Authors

M H Josefsen

N Cook

M DAgostino

F Hansen

M Wagner

K Demnerova

A E Heuvelink

P T Tassios

H Lindmark

V Kmet

M Barbanera

P Fach

S Loncarevic

J Hoorfar

Affiliations

Soon will be listed here.

Abstract

A PCR-based method for rapid detection of food-borne thermotolerant campylobacters was evaluated through a collaborative trial with 12 laboratories testing spiked carcass rinse samples. The method showed an interlaboratory diagnostic sensitivity of 96.7% and a diagnostic specificity of 100% for chicken samples, while these values were 94.2 and 83.3%, respectively, for pig samples.

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