MRNA Expression of Nuclear Receptor RZR/RORalpha, Melatonin Membrane Receptor MT, and Hydroxindole-O-methyltransferase in Different Populations of Human Immune Cells
Overview
Affiliations
We characterized the expression levels of the retinoid Z receptor alpha (RZR alpha), RORalpha mRNA isoforms (RORalpha1, RORalpha2, and RORalpha3), and both melatonin receptor MT1 and hydroxindole-O-methyltransferase (HIOMT) genes. For this purpose, the following human peripheral blood mononuclear cells populations were isolated: monocytes (CD14+ cells), B lymphocytes (CD19+ cells), T helper lymphocytes (CD14(-) CD4+), cytotoxic T lymphocytes (CD56(-) CD8+ cells), and natural killer (NK) lymphocytes (CD56+ cells). PBMCs subsets were obtained by Dynabeads M-450 (Dynal) isolation procedure. We observed a strong gene expression signal for RZRalpha in all subpopulations studied, whereas both RORalpha1 and RORalpha2 transcripts were amplified only in CD8+ cells. Specific signal for RORalpha2 was obtained in all subpopulations studied, but we were not able to detect the RORalpha3 mRNA transcript in human immune cells studied. A weaker signal (especially in CD19+ cells) was also detected in all subsets of cells for the MT1 gene. With regard to HIOMT, a strong signal was achieved among all but one subpopulation of cells; the only exception was CD14+ cells. Thus, in addition to its classical function in the nervous and endocrine system, melatonin could act directly as a paracrine and/or autocrine agent in the human immune system.
Shi Y, Zhu J, Hou C, Li X, Tong Q Aging (Albany NY). 2024; 16(12):10299-10320.
PMID: 38874508 PMC: 11236317. DOI: 10.18632/aging.205930.
Wong Q, Lim J, Ng J, Lim Y, Sio Y, Chew F J Physiol Anthropol. 2024; 43(1):6.
PMID: 38291494 PMC: 10826209. DOI: 10.1186/s40101-024-00356-5.
Melatonin: a modulator in metabolic rewiring in T-cell malignancies.
Rai S, Roy G, Hajam Y Front Oncol. 2024; 13:1248339.
PMID: 38260850 PMC: 10800968. DOI: 10.3389/fonc.2023.1248339.
Melatonin as a Therapy for Preterm Brain Injury: What Is the Evidence?.
Hausler S, Robertson N, Golhen K, van den Anker J, Tucker K, Felder T Antioxidants (Basel). 2023; 12(8).
PMID: 37627625 PMC: 10451719. DOI: 10.3390/antiox12081630.
Huang J, Li Z, Hu Y, Li Z, Xie Y, Huang H J Neuroinflammation. 2022; 19(1):124.
PMID: 35624485 PMC: 9145533. DOI: 10.1186/s12974-022-02477-z.