» Articles » PMID: 15230868

MRNA Expression of Nuclear Receptor RZR/RORalpha, Melatonin Membrane Receptor MT, and Hydroxindole-O-methyltransferase in Different Populations of Human Immune Cells

Overview
Journal J Pineal Res
Publisher Wiley
Specialty Endocrinology
Date 2004 Jul 3
PMID 15230868
Citations 30
Authors
Affiliations
Soon will be listed here.
Abstract

We characterized the expression levels of the retinoid Z receptor alpha (RZR alpha), RORalpha mRNA isoforms (RORalpha1, RORalpha2, and RORalpha3), and both melatonin receptor MT1 and hydroxindole-O-methyltransferase (HIOMT) genes. For this purpose, the following human peripheral blood mononuclear cells populations were isolated: monocytes (CD14+ cells), B lymphocytes (CD19+ cells), T helper lymphocytes (CD14(-) CD4+), cytotoxic T lymphocytes (CD56(-) CD8+ cells), and natural killer (NK) lymphocytes (CD56+ cells). PBMCs subsets were obtained by Dynabeads M-450 (Dynal) isolation procedure. We observed a strong gene expression signal for RZRalpha in all subpopulations studied, whereas both RORalpha1 and RORalpha2 transcripts were amplified only in CD8+ cells. Specific signal for RORalpha2 was obtained in all subpopulations studied, but we were not able to detect the RORalpha3 mRNA transcript in human immune cells studied. A weaker signal (especially in CD19+ cells) was also detected in all subsets of cells for the MT1 gene. With regard to HIOMT, a strong signal was achieved among all but one subpopulation of cells; the only exception was CD14+ cells. Thus, in addition to its classical function in the nervous and endocrine system, melatonin could act directly as a paracrine and/or autocrine agent in the human immune system.

Citing Articles

Mining key circadian biomarkers for major depressive disorder by integrating bioinformatics and machine learning.

Shi Y, Zhu J, Hou C, Li X, Tong Q Aging (Albany NY). 2024; 16(12):10299-10320.

PMID: 38874508 PMC: 11236317. DOI: 10.18632/aging.205930.


Sleep and allergic diseases among young Chinese adults from the Singapore/Malaysia Cross-Sectional Genetic Epidemiology Study (SMCGES) cohort.

Wong Q, Lim J, Ng J, Lim Y, Sio Y, Chew F J Physiol Anthropol. 2024; 43(1):6.

PMID: 38291494 PMC: 10826209. DOI: 10.1186/s40101-024-00356-5.


Melatonin: a modulator in metabolic rewiring in T-cell malignancies.

Rai S, Roy G, Hajam Y Front Oncol. 2024; 13:1248339.

PMID: 38260850 PMC: 10800968. DOI: 10.3389/fonc.2023.1248339.


Melatonin as a Therapy for Preterm Brain Injury: What Is the Evidence?.

Hausler S, Robertson N, Golhen K, van den Anker J, Tucker K, Felder T Antioxidants (Basel). 2023; 12(8).

PMID: 37627625 PMC: 10451719. DOI: 10.3390/antiox12081630.


Melatonin, an endogenous hormone, modulates Th17 cells via the reactive-oxygen species/TXNIP/HIF-1α axis to alleviate autoimmune uveitis.

Huang J, Li Z, Hu Y, Li Z, Xie Y, Huang H J Neuroinflammation. 2022; 19(1):124.

PMID: 35624485 PMC: 9145533. DOI: 10.1186/s12974-022-02477-z.